Plant regeneration from anther derived callus cultures of ginger (Zingiber officinale Rosc.)
DSpice at Indian Institute of Spices Research
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Creator |
SAMSUDEEN, K
NIRMAL BABU, K MINOO, DIVAKARAN RAVINDRAN, P N |
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Date |
2011-12-18T21:38:53Z
2011-12-18T21:38:53Z 2000 |
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Identifier |
http://hdl.handle.net/123456789/779
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Description |
Ginger anthers collected at the uninucleate micros pore stage were subjected to a cold treatment (0°) for 7 d and induced to develop profuse callus on MS medium supplemented with 2-3 mg I-I 2.4-0. PlantIets could be regenerated from these calli on MS medium supplemented with 5-10 mg 1-1 BAP and 0.2 mg 1-1 2,4-0. The regenerated plantlets could be established in soil with 85% success, when they were planted in potting mixture of garden soil. sand and vermiculite in equal proportions and kept in a humid chamber initially for 22-30 d. This is the first report of successful regeneration of plants from ginger anther cultures and the protocol can be used for possible development of androgenic haploids and dihaploids in ginger.
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Format |
444128 bytes
application/pdf |
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Language |
en
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Subject |
Plant regeneration
callus cultures ginger |
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Title |
Plant regeneration from anther derived callus cultures of ginger (Zingiber officinale Rosc.)
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Type |
Article
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