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Replication Data for: The effect of six-weeks advanced oral care on oral microbiome and mycobiome composition in people with dementia living in aged-care facilities

Harvard Dataverse (Africa Rice Center, Bioversity International, CCAFS, CIAT, IFPRI, IRRI and WorldFish)

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Title Replication Data for: The effect of six-weeks advanced oral care on oral microbiome and mycobiome composition in people with dementia living in aged-care facilities
 
Identifier https://doi.org/10.7910/DVN/EFFUI1
 
Creator Bowman, John
 
Publisher Harvard Dataverse
 
Description Microbiome data for study: The effect of six-weeks advanced oral care on oral microbiome and mycobiome composition in people with dementia living in aged-care facilities.

Description. The oral cavities of two sets of participants (elderly, mild dementia, average age 84, in aged care facilities) - control (n=10) and experimental (n=27) - were sampled (swabs of cheeks, gums and tongue). The control group practiced normal oral care. The experimental group had more active oral care implemented (increased tooth brushing and denture cleaning). The active oral care period was maintained for 6 weeks. Samples were taken before the oral care program was instigated and immediately following its cessation.

Metadata:
Gender of participants
Sampling point (pre-intervention, post-intervention)
Oral site sampled - cheeks, gums, tongue
Cohort - control, experimental
Treatment group - control, experimental x pre-intervention, post-intervention
Client ID

Origin of Data: 16S and 18S rRNA amplicon metabarcode sequence data deposited in NCBI Sequence Read Archive under bioprject PRJNA107328. Amplicon survey for bacterial 16S rRNA genes (V3-V4 region, 300 bp pair-end joined) and fungal 18S rRNA genes (V9 region, un-joined since the forward and reverse sequences fully overlap) performed via Illumina MiSeq (total reads 20.5 million and 1.3 million, respectively following data filtration and chimera removal).

Datafiles contain: 1) OTU table of raw read counts following trimming, joining, filtration and chimera removal. Includes relevant metadata for the study. 2) data converted to centered log ratios for downstream beta analysis (PCA, PERMANOVA, CAP etc.).
 
Subject Medicine, Health and Life Sciences
oral health
dementia
 
Date 2024-02-07
 
Contributor Bowman, John