Record Details

Analyzing the Impact of RNA Lipid Aggregates on Fibroblast Reticular Cells via RNA Sequencing In Vitro

Harvard Dataverse (Africa Rice Center, Bioversity International, CCAFS, CIAT, IFPRI, IRRI and WorldFish)

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Title Analyzing the Impact of RNA Lipid Aggregates on Fibroblast Reticular Cells via RNA Sequencing In Vitro
 
Identifier https://doi.org/10.7910/DVN/FBCAGI
 
Creator Mendez-Gomez, Hector
 
Publisher Harvard Dataverse
 
Description Fibroblastic reticular cells (FRCs) were cultured in 6-well plates and treated with lipid particles (NP samples, n = 3 wells) or RNA-loaded lipid particle aggregates (RNA samples, n = 3 wells). 24 hours later, the cells were harvested, and RNA immediately extracted. The RNA samples were then sent to Novogene for library preparation. Sequencing was conducted on the Illumina NovaSeq 6000 platform, utilizing a paired-end 150 bp sequencing approach. The RNA used for treatment was GFP mRNA, obtained from Trilink (cat. L-7601).
 
Subject Medicine, Health and Life Sciences
mRNA, cancer vaccines, lipid-nanoparticles, cancer immunotherapy, glioblastoma, personalized therapy
 
Date 2024-01-09
 
Contributor Mendez-Gomez, Hector