Record Details

Virtual screening and in vitro assay of potential drug like inhibitors from spices against Glutathione-S-Transferase of Meloidogyne incognita

DSpice at Indian Institute of Spices Research

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Field Value
 
Creator ROSANA, O B
DINSHA, MOORKOTH
SHAMINA A
EAPEN, S J
 
Date 2012-02-15T19:12:48Z
2012-02-15T19:12:48Z
2012
 
Identifier Bioinformation 8(7): 319-325 (2012)
0973-2063
http://hdl.handle.net/123456789/804
 
Description Glutathione S-transferases (GSTs) enzymes are critical antioxidant and detoxification system responsible for long-term existence of nematodes in host species. Hence, 16 phytochemicals predicted and reported to have potential nematicidal activity have been docked to GST enzyme of Meloidogyne incognita to assess their binding affinity and inhibitory activity. In vitro effects of these phytochemicals from in silico results have been done for validation of docking studies and efficacy in GST inhibition of following
compounds such as alpha- pinene, alpha- terpineol, beta- caryophyllene, capsaicin, cinnamic acid, citronellol, curcumin, eugenol, geraniol, isoeugenol, linalool, myristicin, neral, NVA (N-vanillylnonanamide), piperine, vanillin have been revealed. Nematode inhibition in vitro bioassay for selected compounds could conclude that maximum mortality was observed with highest concentrations of beta- caryophyllene (78%) followed by eugenol (61.6%), cinnamic acid (55%) and N-vanillylnonanamide (49%). These findings thus suggest that the above phytochemicals could be potentially developed as nematicidal molecules against M.
incognita infections.
 
Format 441927 bytes
application/pdf
 
Language en
 
Publisher Biomedical Informatics
 
Subject Meloidogyne incognita
Docking
Glutathione S-transferase(s)
Phytochemicals
 
Title Virtual screening and in vitro assay of potential drug like inhibitors from spices against Glutathione-S-Transferase of Meloidogyne incognita
 
Type Article