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Identification and application of a candidate gene AhAftr1 for aflatoxin production resistance in peanut seed (Arachis hypogaea L.)

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Relation http://oar.icrisat.org/12535/
https://www.sciencedirect.com/science/article/pii/S2090123223002631
https://doi.org/10.1016/j.jare.2023.09.014
 
Title Identification and application of a candidate gene AhAftr1 for aflatoxin production resistance in peanut seed (Arachis hypogaea L.)
 
Creator Yu, B
Liu, N
Huang, L
Luo, H
Zhou, X
Lei, Y
Yan, L
Wang, X
Chen, W
Kang, Y
Ding, Y
Jin, G
Pandey, M K
Janila, P
Sudini, H K
Varshney, R K
Jiang, H
Liu, S
Liao, B
 
Subject Groundnut
Aflatoxins
 
Description Introduction
Peanut is susceptible to infection of Aspergillus fungi and conducive to aflatoxin contamination, hence developing aflatoxin-resistant variety is highly meaningful. Identifying functional genes or loci conferring aflatoxin resistance and molecular diagnostic marker are crucial for peanut breeding.

Objectives
This work aims to (1) identify candidate gene for aflatoxin production resistance, (2) reveal the related resistance mechanism, and (3) develop diagnostic marker for resistance breeding program.

Methods
Resistance to aflatoxin production in a recombined inbred line (RIL) population derived from a high-yielding variety Xuhua13 crossed with an aflatoxin-resistant genotype Zhonghua 6 was evaluated under artificial inoculation for three consecutive years. Both genetic linkage analysis and QTL-seq were conducted for QTL mapping. The candidate gene was further fine-mapped using a secondary segregation mapping population and validated by transgenic experiments. RNA-Seq analysis among resistant and susceptible RILs was used to reveal the resistance pathway for the candidate genes.

Results
The major effect QTL qAFTRA07.1 for aflatoxin production resistance was mapped to a 1.98 Mbp interval. A gene, AhAftr1 (Arachis hypogaea Aflatoxin resistance 1), was detected structure variation (SV) in leucine rich repeat (LRR) domain of its production, and involved in disease resistance response through the effector-triggered immunity (ETI) pathway. Transgenic plants with overexpression of AhAftr1(ZH6) exhibited 57.3% aflatoxin reduction compared to that of AhAftr1(XH13). A molecular diagnostic marker AFTR.Del.A07 was developed based on the SV. Thirty-six lines, with aflatoxin content decrease by over 77.67% compared to the susceptible control Zhonghua12 (ZH12), were identified from a panel of peanut germplasm accessions and breeding lines through using AFTR.Del.A07.

Conclusion
Our findings would provide insights of aflatoxin production resistance mechanisms and laid meaningful foundation for further breeding programs.
 
Publisher Elsevier B.V. on behalf of Cairo University
 
Date 2023-09-20
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights cc_by_nc_nd
 
Identifier http://oar.icrisat.org/12535/1/Journal%20of%20Advanced%20Research_1-12_2023.pdf
Yu, B and Liu, N and Huang, L and Luo, H and Zhou, X and Lei, Y and Yan, L and Wang, X and Chen, W and Kang, Y and Ding, Y and Jin, G and Pandey, M K and Janila, P and Sudini, H K and Varshney, R K and Jiang, H and Liu, S and Liao, B (2023) Identification and application of a candidate gene AhAftr1 for aflatoxin production resistance in peanut seed (Arachis hypogaea L.). Journal of Advanced Research. pp. 1-12. ISSN 2090-1232