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Comparative diagnostic efficacy of avidin-biotin recombinant nucleoprotein competitive ELISA for serosurveillance and monitoring of peste des petits ruminants in sheep and goats

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Title Comparative diagnostic efficacy of avidin-biotin recombinant nucleoprotein competitive ELISA for serosurveillance and monitoring of peste des petits ruminants in sheep and goats
Not Available
 
Creator Balamurugan V
Bokade PP
Kumar KV
Sowjanya Kumari S
Nagalingam M
Hemadri D
Shome BR
 
Subject ABrC-ELISA PPR
Diagnostic efficacy
Evaluation
Recombinant nucleoprotein
Serosurveillance/monitoring
 
Description Not Available
In this study extensive evaluation of Avidin-Biotin recombinant nucleoprotein competitive ELISA (ABrC-ELISA) was carried out by mass screening of a large number of sera to make use of this assay for serosurveillance and seromonitoring of peste des petits ruminants (PPR) in sheep and goats to evaluate its diagnostic efficacy value and strengthen findings associated with the assay. The recombinant PPR virus (PPRV) nucleoprotein was over-expressed in E. coli, Ni-NTA affinity-purified, and characterized and used as coating diagnostic antigen in ABrC-ELISA, and evaluated using the field sera from animals. On evaluation of the diagnostic performance or efficacy of this assay using the pre-vaccinated and post-vaccinated sera of sheep and goats (n = 1437), the ABrC-ELISA showed a relative diagnostic sensitivity of 87.2% (95% CI: 84.1-90%) and diagnostic specificity of 92.0% (95% CI: 90-93.7%), against well-established existing indigenous H protein-specific PPR competitive ELISA kit with an accuracy of 90.1% (95% CI: 88.5-91.7%) and good or substantial agreement of Cohen's Kappa value of 0.79 ± 0.017 SE (95% CI: 0.76 to 0.82). These findings suggest that the ABrC-ELISA is a potential additional diagnostic tool of a rapid, sensitive, and specific assay for the detection of the PPRV nucleoprotein antibodies in sera of sheep and goats. This PPR Ab Chek kit can be used extensively under field conditions for serosurveillance, and seromonitoring of PPR in sheep and goats at the eradication /post-eradication phase in disease-controlled countries or PPR non-enzootic countries.
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Date 2024-03-05T11:26:38Z
2024-03-05T11:26:38Z
2023-01-01
 
Type Research Paper
 
Identifier 2. Balamurugan, V., Bokade, P.P., Kumar, K.V., Sowjanya Kumari, S., Nagalingam, M., Hemadri, D., Shome, B.R. (2023). Comparative diagnostic efficacy of avidin-biotin recombinant nucleoprotein competitive ELISA for serosurveillance and monitoring of peste des petits ruminants in sheep and goats. Journal of Immunological Methods, 512, 113409.
0022-1759
http://krishi.icar.gov.in/jspui/handle/123456789/81568
 
Language English
 
Relation Not Available;
 
Publisher Elsevier Publishers