Record Details

Preliminary evaluation of Lablab purpureus phytochemicals for anti-BoHV-1 activity using in vitro and in silico approaches

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Preliminary evaluation of Lablab purpureus phytochemicals for anti-BoHV-1 activity using in vitro and in silico approaches
Not Available
 
Creator Bhat SS
Pradeep S
Patil SS
Flores-Holguin N
Glossman-Mitnik D
Frau J
Sommano SR
Ali N
Mohanty M
Shivamallu C
Prasad SK
 
Subject Phytochemical
Evaluation
 
Description Not Available
Lablabpurpureusfrom the Fabaceaefamily has been reportedto haveantiviralpropertiesand used in traditionalmedicalsystemslike ayurvedaand Chinesemedicineand has been employedto treat a variety of illnessesincludingcholera,foodpoisoning,diarrhea,and phlegmaticdiseases.The bovine alphaherpesvirus-1(BoHV-1)isnotoriousfor causingsignificantharm to the veterinaryand agricultureindustries.Theremovalof the contagiousBoHV-1from host organs,particularlyin those reservoircreatures,has requiredthe use of antiviraldrugs that target infectedcells. This studydevelopedLP-CuONPs from methanoliccrude extracts,and FTIR, SEM, and EDXanalyseswere used to confirmtheir formation.SEM analysisrevealedthat the LP-CuONPshad a sphericalshape with particlesizes between22 and 30 nm. Energy-dispersiveX-raypatternanalysisrevealedthe presenceof only copper and oxide ions. By preventingviralcytopathiceffects in the Madin−Darbybovine kidney cell line, the methanolicextract ofLablabpurpureusand LP-CuONPs demonstrateda remarkabledose-dependentanti-BoHV-1actionin vitro. Furthermore,moleculardockingand moleculardynamicssimulationstudiesof bio-activesfromLablabpurpureusagainstthe BoHV-1viral envelopeglycoproteindisclosedeffectiveinteractionsbetweenall phytochemicalsand the protein,althoughkievitonewas found to have the highestbindingaffinity,with thegreatestnumberof interactions,which was also validatedwith moleculardynamicssimulationstudies.Understandingthe chemicalreactivityqualitiesof the four ligandswas taken into considerationfacilitatedby the global and local descriptors,which aimed topredictthe chemicalreactivitydescriptorsof the studiedmoleculesthroughthe conceptualDFT methodology,which, along withADMETfinding,supportthe in vitro and in silico results.
Not Available
 
Date 2024-03-05T12:06:16Z
2024-03-05T12:06:16Z
2023-06-14
 
Type Research Paper
 
Identifier 5. Bhat, S.S., Pradeep, S., Patil, S.S., Flores-Holguín, N., Glossman-Mitnik, D., Frau, J., Sommano, S.R., Ali, N., Mohany, M., Shivamallu, C. and Prasad, S.K. (2023). Preliminary evaluation of Lablab purpureus phytochemicals for anti-BoHV-1 activity using in vitro and in silico approaches. ACS Omega, 2023(8): 22684-22697.
2470-1343
http://krishi.icar.gov.in/jspui/handle/123456789/81571
 
Language English
 
Relation Not Available;
 
Publisher ACS Publishers