Preliminary evaluation of Lablab purpureus phytochemicals for anti-BoHV-1 activity using in vitro and in silico approaches
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Title |
Preliminary evaluation of Lablab purpureus phytochemicals for anti-BoHV-1 activity using in vitro and in silico approaches
Not Available |
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Creator |
Bhat SS
Pradeep S Patil SS Flores-Holguin N Glossman-Mitnik D Frau J Sommano SR Ali N Mohanty M Shivamallu C Prasad SK |
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Subject |
Phytochemical
Evaluation |
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Description |
Not Available
Lablabpurpureusfrom the Fabaceaefamily has been reportedto haveantiviralpropertiesand used in traditionalmedicalsystemslike ayurvedaand Chinesemedicineand has been employedto treat a variety of illnessesincludingcholera,foodpoisoning,diarrhea,and phlegmaticdiseases.The bovine alphaherpesvirus-1(BoHV-1)isnotoriousfor causingsignificantharm to the veterinaryand agricultureindustries.Theremovalof the contagiousBoHV-1from host organs,particularlyin those reservoircreatures,has requiredthe use of antiviraldrugs that target infectedcells. This studydevelopedLP-CuONPs from methanoliccrude extracts,and FTIR, SEM, and EDXanalyseswere used to confirmtheir formation.SEM analysisrevealedthat the LP-CuONPshad a sphericalshape with particlesizes between22 and 30 nm. Energy-dispersiveX-raypatternanalysisrevealedthe presenceof only copper and oxide ions. By preventingviralcytopathiceffects in the Madin−Darbybovine kidney cell line, the methanolicextract ofLablabpurpureusand LP-CuONPs demonstrateda remarkabledose-dependentanti-BoHV-1actionin vitro. Furthermore,moleculardockingand moleculardynamicssimulationstudiesof bio-activesfromLablabpurpureusagainstthe BoHV-1viral envelopeglycoproteindisclosedeffectiveinteractionsbetweenall phytochemicalsand the protein,althoughkievitonewas found to have the highestbindingaffinity,with thegreatestnumberof interactions,which was also validatedwith moleculardynamicssimulationstudies.Understandingthe chemicalreactivityqualitiesof the four ligandswas taken into considerationfacilitatedby the global and local descriptors,which aimed topredictthe chemicalreactivitydescriptorsof the studiedmoleculesthroughthe conceptualDFT methodology,which, along withADMETfinding,supportthe in vitro and in silico results. Not Available |
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Date |
2024-03-05T12:06:16Z
2024-03-05T12:06:16Z 2023-06-14 |
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Type |
Research Paper
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Identifier |
5. Bhat, S.S., Pradeep, S., Patil, S.S., Flores-Holguín, N., Glossman-Mitnik, D., Frau, J., Sommano, S.R., Ali, N., Mohany, M., Shivamallu, C. and Prasad, S.K. (2023). Preliminary evaluation of Lablab purpureus phytochemicals for anti-BoHV-1 activity using in vitro and in silico approaches. ACS Omega, 2023(8): 22684-22697.
2470-1343 http://krishi.icar.gov.in/jspui/handle/123456789/81571 |
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Language |
English
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Relation |
Not Available;
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Publisher |
ACS Publishers
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