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Replication Data for "Functional labeling of individualized postsynaptic neurons using optogenetics and trans-Tango in Drosophila (FLIPSOT)"

Harvard Dataverse (Africa Rice Center, Bioversity International, CCAFS, CIAT, IFPRI, IRRI and WorldFish)

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Title Replication Data for "Functional labeling of individualized postsynaptic neurons using optogenetics and trans-Tango in Drosophila (FLIPSOT)"
 
Identifier https://doi.org/10.7910/DVN/ZHLU5Y
 
Creator Castaneda, Allison
Huda, Ainul
Whitaker, Iona
Reilly, Julianne
Shelby, Grace
Bai, Hua
Ni, Lina
 
Publisher Harvard Dataverse
 
Description A population of neurons interconnected by synapses constitutes a neural circuit, which performs specific functions upon activation. It is essential to identify both anatomical and functional entities of neural circuits to comprehend the components and processes necessary for healthy brain function and the changes that characterize brain disorders. To date, few methods are available to study these two aspects of a neural circuit simultaneously. In this study, we developed FLIPSOT, or functional labeling of individualized postsynaptic neurons using optogenetics and trans-Tango. FLIPSOT uses (1) trans-Tango to access postsynaptic neurons genetically, (2) optogenetic approaches to activate (FLIPSOTa) or inhibit (FLIPSOTi) postsynaptic neurons in a random and sparse manner, and (3) fluorescence markers tagged with optogenetic genes to visualize these neurons. Therefore, FLIPSOT allows using a presynaptic driver to identify the behavioral function of individual postsynaptic neurons. It is readily applied to identify functions of individual postsynaptic neurons and has the potential to be adapted for use in mammalian circuits.
 
Subject Medicine, Health and Life Sciences
FLIPSOT, optogenetics, GtACR, CsChrimson, FLP/FRT, trans-Tango, Drosophila, thermotaxis, heating cells, projection neurons
 
Date 2024-02-15
 
Contributor Ni, Lina