Record Details

Archival chromatin sequencing in eastern water dragon museum specimens

CSIRO RDS Repository

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Title Archival chromatin sequencing in eastern water dragon museum specimens
 
Creator Erin Hahn
 
Subject Epigenetics (incl. genome methylation and epigenomics)
Critical heritage, museum and archive studies
 
Description The data consist of 150 bp paired-end Illumina sequencing reads from chromatin conformation assay of 5 archival eastern water dragon (Intellagama lesueurii lesueurii) specimens and whole genome sequencing of three contemporary specimens. The archival specimens, collected between 1905 and 2001, were spirit-preserved and formalin-exposed. Archival liver tissue was dissected and subjected to MNase treatment heavily optimised for archival tissues. The contemporary specimens had been euthanised due to injury in accordance with Queensland Department of Environment and Sciences permit WA0038029 (Australian Ethics Committee number ANA20161, University of Sunshine Coast). From these fresh specimens, we extracted approximately 5 mg of liver tissue per specimen with the Qiagen AllPrep kit. The extracted or enriched DNA was then prepared for sequencing using an IDT xGEN Prism library prep kit.

The data were generated as part of a collaboration with Celine Frere at the University of Queensland.
 
Publisher CSIRO
 
Contributor Jiri Stiller
Marina Alexander
Alicia Grealy
Jen Taylor
Nicola Kent
Celine Frere
Clare Holleley
 
Date 2023-12-10
 
Type —
 
Format —
 
Identifier csiro:59757
 
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