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A lateral flow assay for the detection of Leptospira lipL32 gene using CRISPR technology

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Title A lateral flow assay for the detection of Leptospira lipL32 gene using CRISPR technology
Not Available
 
Creator Natarajan S
Joseph J
Balamurugan V
Estrela P
 
Subject Leptospira
CRISPR
dFnCas9
lateral flow device
 
Description Not Available
The clinical manifestation of leptospirosis is often misdiagnosed as other febrile illnesses such as dengue. Therefore, there is an urgent need for a precise diagnostic tool at the field level to detect the pathogenic Leptospira lipL32 gene at the molecular level for prompt therapeutic decisions. Quantitative polymerase chain reaction (qPCR) is widely used as the primary diagnostic tool, but its applicability is limited by high equipment cost and the lack of availability in every hospital, especially in rural areas where leptospirosis mainly occurs. Here, we report the development of a CRISPR dFnCas9-based quantitative lateral flow immunoassay to detect the lipL32 gene. The developed assay showed superior performance regarding the lowest detectable limit of 1 fg/mL. The test is highly sensitive and selective, showing that leptospirosis diagnosis can be achieved with a low-cost lateral flow device.
Not Available
 
Date 2024-03-06T10:52:03Z
2024-03-06T10:52:03Z
2023-07-20
 
Type Research Paper
 
Identifier Natarajan S, Joseph J, Vinayagamurthy B, Estrela P. A Lateral Flow Assay for the Detection of Leptospira lipL32 Gene Using CRISPR Technology. Sensors. 2023; 23(14):6544. https://doi.org/10.3390/s23146544
1424-8220
http://krishi.icar.gov.in/jspui/handle/123456789/81598
 
Language English
 
Relation Not Available;
 
Publisher MDPI Publishers