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Analysis of genetic diversity of cytb gene from Babesia gibsoni isolates from naturally infected dogs in Karnataka, India

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Title Analysis of genetic diversity of cytb gene from Babesia gibsoni isolates from naturally infected dogs in Karnataka, India
Not Available
 
Creator Vijayakumar, LK
Chikkachowdappa PG
Venkatappa MH
Gowda AKJ
Jacob SS
Mani S
Manjunatha Reddy GB
 
Subject Babesia gibsoni
Cytochrome b gene
Dogs
Genetic diversity
Phylogenetic analysis
 
Description Not Available
Purpose: The study aimed to investigate genetic diversity in Babesia gibsoni, the causative agent of canine babesiosis, and to assess the presence of atovaquone-resistant isolates in naturally infected dogs.

Methods: A total of 24 blood samples confirmed for B. gibsoni infection was subjected to PCR amplification and sequencing based on cytb gene. Genetic characterization of B. gibsoni as well as attempts to detect the point mutation rendering atovaquone resistance was carried out based on the analysis of nucleotide sequence of cytb gene using bioinformatics software.

Results: The findings indicated that the B. gibsoni isolates in the investigation exhibited a high nucleotide identity with the Asian genotype, ranging from 98.41 to 98.69%. Notably, none of the isolates carried cytb gene variants associated with atovaquone resistance. Phylogenetic analysis revealed clustering of most isolates with those from Japan and China, except for one isolate forming a distinct subclade. Haplotype network analysis indicated a high diversity with 22 distinct haplotypes among the B. gibsoni isolates, emphasizing the genetic variability within the studied population.

Conclusion: In conclusion, the cytb gene exhibited remarkable conservation among the twenty-four B. gibsoni isolates studied and the study represents the first genetic diversity assessment of B. gibsoni using the cytb gene in dogs from India. These findings shed light on the genetic characteristics of B. gibsoni in the region and provide valuable insight for addressing the challenges posed by this life-threatening disease in dogs.
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Date 2024-03-06T16:42:11Z
2024-03-06T16:42:11Z
2023-12-22
 
Type Research Paper
 
Identifier 39. Vijaykumar, L.K., Chikkachowdappa, P.G., Venkatappa, M.H., Gowda, A.K.J., Jacob, S.S., Mani, S. and Bayyappa, M.R.G. (2023). Analysis of genetic diversity of cytb gene from Babesia gibsoni isolates from naturally infected dogs in Karnataka, India. Acta Parasitologica, 1-9.
18961851
http://krishi.icar.gov.in/jspui/handle/123456789/81615
 
Language English
 
Relation Not Available;
 
Publisher Springer Nature