Record Details

Comparative effectiveness of inter-simple sequence repeat and randomly amplified polymorphic DNA markers to study genetic diversity of Indian Garcinia

DSpice at Indian Institute of Spices Research

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Field Value
 
Creator UTPALA PARTHASARATHY
NANDAKISHORE, O P
NIRMAL BABU, K
SENTHIL KUMAR
PARTHASARATHY, V A
 
Date 2013-06-23T16:47:33Z
2013-06-23T16:47:33Z
2013
 
Identifier African journal of biotechnology Vol.12(46):6443-6451(2013)
http://hdl.handle.net/123456789/1030
 
Description A study to compare the effectiveness of inter-simple sequence repeats (ISSR) and randomly amplified polymorphic DNA (RAPD) profiling was carried out with a total of 65 DNA samples using 12 species of Indian Garcinia. ISSR and RAPD profiling were performed with 19 and 12 primers, respectively. ISSR markers generated a total number of 156 bands with 92 polymorphic bands, while RAPD markers produced a total of 134 bands with 80 polymorphic bands. Percentage of polymorphic loci in RAPD
profiling was 60.4% while in ISSR profiling, it was 59.3%. Heterogeneity index was similar for the markers, 0.86 for ISSR and 0.89 for RAPD, indicating that both the marker systems are effective in determining polymorphism in Garcinia. ISSR markers showed clear distinction among the species
whereas RAPD markers showed segregation based on geographical location as well as species based.
 
Format 523460 bytes
application/pdf
 
Language en
 
Publisher Academic Journals
 
Subject Garcinia
Genetic diversity
Inter-simple sequence repeats
Randomly amplified polymorphic DNA
Principal component analysis
 
Title Comparative effectiveness of inter-simple sequence repeat and randomly amplified polymorphic DNA markers to study genetic diversity of Indian Garcinia