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Genetic diversity analysis of Myristica and related genera using RAPD and ISSR markers

DSpice at Indian Institute of Spices Research

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Field Value
 
Creator SHEEJA, T E
SABEESH, C
SHABNA, O V
SHALINI, R S
KRISHNAMOORTHY, B
 
Date 2014-02-22T06:54:26Z
2014-02-22T06:54:26Z
2013
 
Identifier Journal of Spices and Aromatic Crops 22 (1) : 38–46 (2013)
http://hdl.handle.net/123456789/1071
 
Description Genetic diversity among seven species of Myristica, two of its related genera and an unidentified species was analyzed using 46 PCR markers (30 RAPD and 16 ISSR). This is the first study on molecular genetic diversity of the rare, endangered and endemic Myristica species and its related genera. RAPD and ISSR analyses yielded 497 and 262 bands with 98.1% and 97.3% polymorphism,respectively. By combining markers, a total of 759 bands were detected of which 743 (97.8%) were
polymorphic with an average of 16.1 bands per primer. High level of existing genetic variability was evident from the high percentage of polymorphism. Combined analysis of RAPD and ISSR markers resulted in better distinction of species. The mean polymorphic information content (PIC) indicated that both the marker systems are effective in detecting polymorphism either
individually or in combination. Similarity coefficient (Jaccards) varied from 0.22 to 0.62 when markers were combined and the pattern was similar to RAPD with a high Mantel matrix correlation (r=0.95). Principal Coordinates Analysis (PCA) conformed to cluster analyses. First
three most informative PC components explained 51.1%, 49.3% and 46.5% of total variation. A maximum similarity of (63%) was observed between Gymnocranthera canarica and the unidentified species of Myristica. Knema andamanica and Myristica prainii were found to be the most distinct (17.7%).Similarities at molecular level were close to either the morphological traits (mace and fruit/seed characters) or the geographical location. Species specific bands could be identified from all the accessions under study, which has the potential for development into SCAR (Sequence Characterised Amplified Region) markers for genotype fingerprinting or development of specific DNA probes for identification and authentication.
 
Format 957253 bytes
application/pdf
 
Language en
 
Publisher Indian Society for Spices
 
Subject nutmeg
UPGMA
genetic variation
endemic
endangered
phylogeny
 
Title Genetic diversity analysis of Myristica and related genera using RAPD and ISSR markers
 
Type Article