Record Details

Reverse transcription loop-mediated isothermal amplification assay for rapid and sensitive detection of Banana bract mosaic virus in cardamom (Elettaria cardamomum)

DSpice at Indian Institute of Spices Research

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Field Value
 
Creator SILJO, A
BHAT, A I
 
Date 2014-06-05T06:37:47Z
2014-06-05T06:37:47Z
2014
 
Identifier European Journal of Plant Pathology 138:209–214(2014)
http://hdl.handle.net/123456789/1117
 
Description Cardamom being a perennial and propagated
vegetatively, Banana bract mosaic virus (BBrMV) in cardamom spreads mainly through infected material.A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for easy
and quick detection of the virus. The following conditions proved optimal for amplification: 4 mM of magnesium sulphate, 1.2 M of betaine, 65 °C, and 1 h of
reaction time. The results were assessed visually by turbidity and green fluorescence (induced by adding manganese chloride and calcein) in the reaction tube and also by gel electrophoresis. The assay successfully
detected the virus in infected plants whereas no cross reaction was recorded with healthy plants. The detection limit for RT-LAMP was up to 100 times that for
conventional RT-PCR and on a par with that for realtime RT-PCR. The assay was validated by testing field samples of cardamom plants from different cardamom growing tracts in Kerala, India.
 
Format 100092 bytes
application/pdf
 
Language en
 
Publisher Springer
 
Subject RT-LAMP
Sensitivity
RT-PCR
Real-time RT-PCR
Validation
 
Title Reverse transcription loop-mediated isothermal amplification assay for rapid and sensitive detection of Banana bract mosaic virus in cardamom (Elettaria cardamomum)
 
Type Article