Record Details

Serological Studies and Polyclonal Antiserum Production against the Nucleocapsid Protein of Mungbean Tospovirus from New Delhi

DSpice at Indian Institute of Spices Research

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Field Value
 
Creator THIEN, HO XUAN
BHAT, A I
JAIN, R K
 
Date 2014-06-19T07:24:06Z
2014-06-19T07:24:06Z
2002
 
Identifier Omonrice 10: 60-66 (2002)
http://hdl.handle.net/123456789/1122
 
Description Natural infection of Tospovirus on mungbean was recorded in New Delhi, India.
Symptomatic mungbean samples reacted positively with Groundnut bud necrosis
virus (GBNV) and Watermelon silver mottle virus (WSMV) antisera in direct
antigen-coated enzyme-linked immunosorbent assay. The virus was maintained in
cowpea which served as a propagation host. The nucleocapsid (N) protein of the
virus was purified. The polyclonal antiserum against the N protein with homologous
titer 1:512 was produced. The antiserum could detect Tospovirus isolates from
cowpea, potato and tomato colleted from different locations.
 
Format 242109 bytes
application/pdf
 
Language en
 
Subject mungbean
Tospovirus
DAC-ELISA
antiserum production
 
Title Serological Studies and Polyclonal Antiserum Production against the Nucleocapsid Protein of Mungbean Tospovirus from New Delhi
 
Type Article