Record Details

Mungbean Necrosis disease caused by a strain of Groundnut Bud Necrosis Virus

DSpice at Indian Institute of Spices Research

View Archive Info
 
 
Field Value
 
Creator THIEN, HO XUAN
BHAT, A I
JAIN, R K
 
Date 2014-06-19T07:30:45Z
2014-06-19T07:30:45Z
2003
 
Identifier Omonrice 11: 110-116 (2003)
http://hdl.handle.net/123456789/1123
 
Description Tospovirus infection on mungbean (up to 70%) was noticed at Indian Agricultural
Research Institute experimental farm, New Delhi, in different varietal trials.
Symptoms under field conditions included necrosis of plant parts such as leaves,
stems, petioles, growing points, buds and pods. Symptomatic mungbean plants
showed positive reaction with Groundnut bud necrosis virus (GBNV) and Watermelon silver mottle virus (WSMV) antisera in direct antigen-coated enzyme linked immunosorbent assay. Mungbean Tospovirus was easily transmitted to cowpea cv. Pusa Komal, which could be used as a diagnostic host. The virus was also mechanically transmitted to different plant species belonging to families
Fabaceae, Cucurbitaceae and Solanaceae. The nucleocapsid (N) gene of the virus was amplified, cloned and sequenced (GenBank Accession number AF515818). The sequenced region contained an ORF of 831 nucleotides that could potentially code for N protein of 276 amino acids. Comparative sequence analyses revealed that the N gene shared 97% and 99% sequence identity with GBNV at nucleotide and amino acid levels respectively, suggesting the Tospovirus isolate from mungbean to be a strain of GBNV.
 
Format 234418 bytes
application/pdf
 
Language en
 
Subject Tospovirus
mungbean
nucleocapsid gene
Groundnut bud necrosis
 
Title Mungbean Necrosis disease caused by a strain of Groundnut Bud Necrosis Virus
 
Type Article