Record Details

Random amplified polymorphic DNA technique for detection of plant based adulterants in chilli powder (Capsicum annuum)

DSpice at Indian Institute of Spices Research

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Field Value
 
Creator DHANYA, K
SYAMKUMAR, S
JALEEL, K
SASIKUMAR, B
 
Date 2014-07-10T05:00:59Z
2014-07-10T05:00:59Z
2008
 
Identifier Journal of Spices and Aromatic Crops 17 (2) : 75-81 (2008)
0971-3328
http://hdl.handle.net/123456789/1129
 
Description The present study reports a modified protocol for isolation of good quality DNA from chilli powder and its probable adulterants (dried red beet pulp, almond shell dust and powdered Ziziphus nummularia fruits) and the utility of RAPD primers for detection of adulterants in marketed chilli powder. Selected RAPD primers, which produced adulterant specific bands in
simulated samples were used for analyzing market samples of chilli powder. Out of the six market samples analyzed, one sample amplified Z. nummularia specific band indicating the occurrence of adulteration in market samples. All the market samples tested were free from dried red beet pulp or almond dust adulteration.
 
Format 1649591 bytes
application/pdf
 
Language en
 
Publisher Indian Society for Spices
 
Subject adulterant detection
chilli powder
DNA isolation
PCR
RAPD
 
Title Random amplified polymorphic DNA technique for detection of plant based adulterants in chilli powder (Capsicum annuum)
 
Type Article