Random amplified polymorphic DNA technique for detection of plant based adulterants in chilli powder (Capsicum annuum)
DSpice at Indian Institute of Spices Research
View Archive InfoField | Value | |
Creator |
DHANYA, K
SYAMKUMAR, S JALEEL, K SASIKUMAR, B |
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Date |
2014-07-10T05:00:59Z
2014-07-10T05:00:59Z 2008 |
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Identifier |
Journal of Spices and Aromatic Crops 17 (2) : 75-81 (2008)
0971-3328 http://hdl.handle.net/123456789/1129 |
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Description |
The present study reports a modified protocol for isolation of good quality DNA from chilli powder and its probable adulterants (dried red beet pulp, almond shell dust and powdered Ziziphus nummularia fruits) and the utility of RAPD primers for detection of adulterants in marketed chilli powder. Selected RAPD primers, which produced adulterant specific bands in simulated samples were used for analyzing market samples of chilli powder. Out of the six market samples analyzed, one sample amplified Z. nummularia specific band indicating the occurrence of adulteration in market samples. All the market samples tested were free from dried red beet pulp or almond dust adulteration. |
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Format |
1649591 bytes
application/pdf |
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Language |
en
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Publisher |
Indian Society for Spices
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Subject |
adulterant detection
chilli powder DNA isolation PCR RAPD |
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Title |
Random amplified polymorphic DNA technique for detection of plant based adulterants in chilli powder (Capsicum annuum)
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Type |
Article
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