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Amperometric biosensor for quantification of Indole 3-acetic acid in water and salt stressed germinating seeds of corn

DSpice at Indian Institute of Spices Research

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Field Value
 
Creator KRISHNAMURTHY, K S
DIGGS, A
PORTERFIELD, D M
 
Date 2014-07-24T08:56:16Z
2014-07-24T08:56:16Z
2013
 
Identifier Communications in Soil Science & Plant Analysis 44 (11):1749 -1763 (2013)
0010-3624
http://hdl.handle.net/123456789/1145
 
Description The amperometric biosensors are known to be sensitive, reliable and cheap instrument for the analysis of environmental bio-ingredients. Recently, amperometric biosensors have been utilized to quantify the endogenous flux of plant hormone, indole 3-acetic acid (IAA) in vivo. In the present study, modified platinum microelectrode with enhanced surface area and thus the efficiency was used for detection of IAA extracted from corn seedlings. Electrode was modified through application of a layer of plantinum black, followed by silanization overnight and coatiing of multi walled carbon nanotubes over it. A BASi cell stand was used for electroanalytical characterization of sensor performance as well as for IAA standard curve
calibration and detection of IAA extracted from corn plant samples using a three electrode scheme (reference, sensing and auxillary). IAA was quantified using this biosensor in water and salt stressed germinating seedlings of corn and was compared with the control. IAA was also
quantified in the same samples using the much established ELISA protocol to compare both the methods and the values obtained from both the methods were comparable and in fact, biosensor method showed slightly higher values. For the first time, amperometric biosensor has been utilized for IAA quantification in plant samples in vitro. Also, the method is simple, cheap and
no elaborate procedures are involved Compared to elaborate purification procedure required for HPLC detection or tedious and time consuming antibody development needed for quantification through ELISA. Both HPLC and ELISA methods are costly also. Hence biosensor method can be effectively utilized for simple, cheap and reliable detection of IAA extracted from plant samples. The results of the stress study clearly indicated that root and shoot elongation growth had significant positive correlation with IAA content. Water and salt stress reduced the root and shoot growth which may be due to overaccumulation of IAA per unit weight, resulting in inhibition of their elongation growth.
 
Format 579032 bytes
application/pdf
 
Language en
 
Publisher Taylor & Francis
 
Subject Amperometric biosensor
Indole 3-acetic acid
corn
plant hormone
water
salt
 
Title Amperometric biosensor for quantification of Indole 3-acetic acid in water and salt stressed germinating seeds of corn
 
Type Article