ICAR Research Data Repository for Knowledge Management
Figure 2 Time-dependent digestion of plasmids with two PaqCI target sites indicate a sequential cleavage profile
Harvard Dataverse (Africa Rice Center, Bioversity International, CCAFS, CIAT, IFPRI, IRRI and WorldFish)
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| Title |
Figure 2 Time-dependent digestion of plasmids with two PaqCI target sites indicate a sequential cleavage profile
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| Identifier |
https://doi.org/10.7910/DVN/62YXDO
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| Creator |
Kennedy, Madison
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| Publisher |
Harvard Dataverse
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| Description |
The reaction contained 1 ug substrate DNA and 2 units/ug PaqCI endonuclease in rCutSmart buffer at 37C. Digest time points were quenched at 0.25 min, 0.5 min, 1 min, 3 min, 5 min, 10 min, 30 min, and 60 minutes with 1 ug dual site substrate and 2 units/ug PaqCI as indicated above each lane. The mobilities of the super coil (SC) and the cleaved linear (Lin, Lin1 and Lin2) forms of the plasmid are indicated in the middle. Below the gels is a cartoon of the substrate and reaction products shown in the gel.
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| Subject |
Chemistry
Medicine, Health and Life Sciences |
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| Contributor |
Kennedy, Madison
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