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Evaluation of genetic diversity of Ralstonia solanacearum causing bacterial wilt of ginger using REP–PCR and PCR–RFLP

DSpice at Indian Institute of Spices Research

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Field Value
 
Creator KUMAR, A
SARMA, Y R
ANANDARAJ, M
 
Date 2014-08-04T05:26:25Z
2014-08-04T05:26:25Z
2004
 
Identifier Current Science 87 (11): 1555-1561(2004)
http://hdl.handle.net/123456789/1153
 
Description Thirty-three strains of Ralstonia solanacearum Yabuuchi (Smith) isolated from ginger, paprika, chilli, tomato, Chromolaena and potato from Kerala, Karnataka, West Bengal and Assam in India, were henotypically
and genotypically characterized. Phenotypic characterization for biovar revealed the predominance of biovar 3 in India. Molecular analysis by REP–PCR, ITS–PCR and RFLP–PCR classified the strains into three
clusters at 70% similarity, where ginger strains are grouped in Clusters I and II. Strains from potato (biovar 2) clustered in the III cluster. Molecular analysis
also revealed that ginger strains isolated from different locations during different years had 100% similarity according to Dice’s coefficient. The analysis further
revealed that the genetic diversity of Ralstonia is very low within ginger, confirming that the pathogen population
is of clonal lineage and is distributed through ‘rhizome transmission’ of the inoculum between locations and also between seasons within the locality.
 
Format 131993 bytes
application/pdf
 
Language en
 
Subject Ginger
Ralstonia solanacearum
bacterial wilt
genetic diversity
REP–PCR
PCR–RFLP
 
Title Evaluation of genetic diversity of Ralstonia solanacearum causing bacterial wilt of ginger using REP–PCR and PCR–RFLP
 
Type Article