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Comparative study of pathogenesis-related protein 5 (PR5) of different Zingiberaceae species

DSpice at Indian Institute of Spices Research

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Field Value
 
Creator Prasath, D
Amruta, Balagopal
Vijay, Mahantesh
Rosana, O B
Jayasankar, S 1
Anandaraj, M
 
Date 2014-12-09T06:25:00Z
2014-12-09T06:25:00Z
2014
 
Identifier Indian Journal of Biotechnology Vol 13: 178-185, 2014
0975-0967
http://hdl.handle.net/123456789/1198
 
Description Pathogenesis-related protein 5 (PR5) is a family of proteins that are induced by different phytopathogens in many plants
and share significant sequence similarity with thaumatin. In the present study, 8 putative PR5 genes were cloned from
different species of Zingiberaceae viz.,
Alpinia luteocarpa Curcuma amada ,. aromatica
,C. longa,Elettaria cardamomum ,Hedychium coronarium , Zingiber officinale and Z. zerumbet.Deduced sequences encode precursor proteins of 215 to 230 amino acid residues and share high homology with a number of other PR5 genes. The phylogenetic analysis of
all the PR5s of Zingiberaceae species showed that all of them share significant evolutionary history. The secondary and 3-D structure comparison with 3 known PR5s (CaPR5,Zo PR5 &ZzPR5) revealed many striking similarities. In addition, we
also generated models of their 3-D structure, the docking interactions between PR5 and the ligand β-(1,4)-D-glucan, and the analysis of various protein properties, which has helped in determining the efficiency of PR5 proteins. These analyses further led to the identification of putative antimicrobial domains in these PR5s, which remain conserved.
 
Format 862512 bytes
application/pdf
 
Language en
 
Publisher NISCAIR, New Delhi
 
Subject Disease resistance
3-D modeling
pathogenesis
pathogenesis-related proteins (PR5)
Zingiberaceae
 
Title Comparative study of pathogenesis-related protein 5 (PR5) of different Zingiberaceae species
 
Type Article