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Interaction between host plant resistance and biological activity of Bacillus thuringiensis in managing the pod borer Helicoverpa armigera in chickpea

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Relation http://oar.icrisat.org/19/
http://dx.doi.org/10.1016/j.cropro.2011.03.018
 
Title Interaction between host plant resistance and biological activity of Bacillus
thuringiensis in managing the pod borer Helicoverpa armigera in chickpea
 
Creator Surekha Devi, V
Sharma, H C
Arjuna Rao, P
 
Subject Chickpea
 
Description The legume pod borer, Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) has developed
high levels of resistance to conventional insecticides, and therefore, efforts are being made to develop
transgenic chickpea expressing toxin genes from the bacterium, Bacillus thuringiensis (Bt) for controlling
this pest. However, there is an apprehension that acid exudates in chickpea might interfere with biological
activity of Bt. Therefore, we studied the biological activity of Bt (BiolepR) on four chickpea genotypes
with different levels of resistance to H. armigera under field conditions, and by incorporating lyophilized
lead and pod tissue into the artificial diet with and without Bt. The pH of the acid exudates varied from
2.1 to 2.90, and malic and oxalic acid were the major components of the acid exudates in different
chickpea genotypes. There was no survival of H. armigera larvae in chickpea plants spayed with 0.1, 0.2
and 0.5% of Bt. There was a significant reduction in larval survival, larval and pupal weights and
fecundity, and prolongation of larval and pupal periods in chickpea plots sprayed with Bt (0.05%) as
compared to the unsprayed plants. Biological activity of Bt was lower on artificial diets with leaf or pod
powder of chickpea genotypes, which might be because of a low intake of Bt toxins due to antifeedant
effects of acid exudates in the chickpea or reduction in biological activity of Bt due to the interaction of
biochemical constituents in chickpea with the Bt toxins. Larval survival, larval and pupal weights,
pupation and adult emergence were significantly lower on diets with leaf or pod powder of the H.
armigera-resistant genotypes than on the susceptible check. Chickpea genotypes with resistance to H.
armigera acted in concert with Bt to cause adverse effects on the survival and development of this insect.
The results suggested that development of transgenic chickpeas expressing toxin genes form Bt will be
quite effective for controlling of the pod borer, H. armigera.
 
Publisher Elsevier
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
application/pdf
 
Language en
en
 
Rights —
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Identifier http://oar.icrisat.org/19/1/HCSharma.pdf
http://oar.icrisat.org/19/4/Chickpea_Bt_int_Crop_Prot_11.pdf
Surekha Devi, V and Sharma, H C and Arjuna Rao, P (2011) Interaction between host plant resistance and biological activity of Bacillus thuringiensis in managing the pod borer Helicoverpa armigera in chickpea. Crop Protection, 30 (8). pp. 962-969. ISSN 0261-2194