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Labcode 5A- HIV neutralization testing with PBMC

Harvard Dataverse (Africa Rice Center, Bioversity International, CCAFS, CIAT, IFPRI, IRRI and WorldFish)

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Title Labcode 5A- HIV neutralization testing with PBMC
 
Identifier https://doi.org/10.7910/DVN/AW0VXC
 
Creator Lindsay Wieczorek
 
Publisher Harvard Dataverse
 
Description This protocol describes a HIV neutralisation assay in the microtiter plate format, that measures reduction in virus infectious titer mediated by exposure to polyclonal or monoclonal reagents. Virus infection in donor PHA-stimulated peripheral blood mononuclear cells (PBMC) is assessed by quantitative ELISA of HIV-1 p24 antigen in PBMC culture supernatants.
 
Subject Medicine, Health and Life Sciences
 
Contributor sheik-khalil, enas