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Evaluation of bacteria isolated from rice rhizosphere for biological control of charcoal rot of sorghum caused by Macrophomina phaseolina (Tassi) Goid.

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Relation http://oar.icrisat.org/39/
http://dx.doi.org/10.1007/s11274-010-0579-0
 
Title Evaluation of bacteria isolated from rice rhizosphere for biological control of charcoal rot of sorghum caused by Macrophomina phaseolina (Tassi) Goid.
 
Creator Gopalakrishnan, S
Humayun, P
Kiran, B K
Kannan, G K
Sree Vidya, M
Deepthi, K
Rupela, Om
 
Subject Sorghum
 
Description A total of 360 bacteria, isolated from the rhizospheres of a system of rice
intensification (SRI) fields, were characterized for the production of siderophore,
fluorescence, indole acetic acid (IAA), hydrocyanic acid (HCN) and solubilization of
phosphorus. Of them, seven most promising isolates (SRI-156, -158, -178, -211, -229, -305
and -360) were screened for their antagonistic potential against Macrophomina phaseolina
(causes charcoal rot in sorghum) by dual culture assay, blotter paper assay and in greenhouse.
All the seven isolates inhibited M. phaseolina in dual culture assay, whereas six isolates
solubilized phosphorous (except SRI-360), all seven produced siderophore, four produced
fluorescence (except SRI-178, -229 and -305), six produced IAA (except SRI-305) and five
produced HCN (except SRI-158 and -305). In the blotter paper assay, no charcoal rot
infection was observed in SRI-156-treated sorghum roots, indicating complete inhibition of
the pathogen, while the roots treated with the other isolates showed 49−76% lesser charcoal
rot infection compared to the control. In the antifungal activity test (in green house on
sorghum), all the isolates increased shoot dry mass by 15−23% and root dry mass by
15−20% (except SRI-158 and -360), over the control. In order to confirm the plant growthpromoting
(PGP) traits of the isolates, the green house experiment was repeated but, in the
absence of M. phaseolina. The results further confirmed the PGP traits of the isolates as
evidenced by increases in shoot and root dry mass, 22−100% and 5−20%, respectively, over
the control. The sequences of 16S rDNA gene of the isolates SRI-156, -158, -178, -211, -229,
-305 and -360 were matched with Pseudomonas plecoglossicida, Brevibacterium antiquum,
Bacillus altitudinis, Enterobacter ludwigii, E. ludwigii, Acinetobacter tandoii and P.
monteilii, respectively in BLAST analysis. This study indicates that the selected bacterial
isolates have the potential for PGP and control of charcoal rot disease in sorghum.
 
Publisher Springer
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
application/pdf
 
Language en
en
 
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Identifier http://oar.icrisat.org/39/1/Gopalakrishnan_et_al_2010-2-6.pdf
http://oar.icrisat.org/39/4/WJMB_27_6_1313-1321_2011.pdf
Gopalakrishnan, S and Humayun, P and Kiran, B K and Kannan, G K and Sree Vidya, M and Deepthi, K and Rupela, Om (2011) Evaluation of bacteria isolated from rice rhizosphere for biological control of charcoal rot of sorghum caused by Macrophomina phaseolina (Tassi) Goid. World Journal of Microbiology and Biotechnology, 27 (6). pp. 1313-1321. ISSN 09593993