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Isolation and Characterization of Baculoviruses from Three Major Lepidopteran Pests in the Semi-Arid Tropics of India

OAR@ICRISAT

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Relation http://oar.icrisat.org/44/
http://dx.doi.org/10.1007/s13337-011-0029-0
 
Title Isolation and Characterization of Baculoviruses from Three
Major Lepidopteran Pests in the Semi-Arid Tropics of India
 
Creator Sridhar Kumar, Ch
Ranga Rao, G V
Sireesha, K
Kumar, P L
 
Subject Entomology
 
Description Baculoviruses were isolated from three major
lepidopteran pests, Helicoverpa armigera, Spodoptera litura
and Amsacta albistriga in the semi-arid tropics during
natural epizootic conditions at ICRISAT fields, Patancheru,
Andhra Pradesh, India. Biological, morphological and
biochemical analysis identified these isolates as Nucleopolyhedroviruses
(NPVs). Scanning electron microscopy of
the occlusion bodies (OBs) purified from diseased larvae
revealed polyhedral particles of size approximately
0.5–2.5 lm [Helicoverpa armigera Nucleopolyhedrovirus
(HearNPV)], 0.9–2.92 lm [Spodoptera litura Nucleopolyhedrovirus
(SpltNPV)] and 1.0–2.0 lm [Amsacta albistriga
Nucleopolyhedrovirus (AmalNPV)] in diameter. Transmission
electron microscopy of thin sections of OBs of the three
isolates revealed up to 5–8 multiple bacilliform shaped
particles packaged within a single viral envelope. The
dimensions of these particles were 277.7 9 41.6 nm for
HearNPV, 285.7 9 34.2 nm for SpltNPV and 228.5 9
22.8 nm for AmalNPV. Each of HearNPV and AmalNPV
contained up to 6 nucleocapsids and SpltNPV contained up
to 7 nucleocapsids per envelope. The estimated molecular
weights of the purified OB (polyhedrin) protein of the three
NPVs were 31.29–31.67 kDa. Virus yield (OBs/larva) was
5.18 ± 0.45 9 109 for HearNPV, 5.73 ± 0.17 9 109 for
SpltNPV and 7.90 ± 0.54 9 109 for AmalNPV. The LC50
values of various NPVs against 2nd and 3rd instar larvae
indicated 2.30 9 104 and 1.5 9 105 OBs/ml for HearNPV,
3.5 9 104 and 2.4 9 105 OBs/ml for SpltNPV and 5.6 9
104 and 3.96 9 105 OBs/ml for AmalNPV. The lethal time
required to cause 50% mortality (LT50) for these three species
were also defined. This study has shown that the NPVs
infecting three major lepidopteran pests in India are multiple
NPVs, and they have good potential to use as biocontrol
agents against these important pests.
 
Publisher Indian Virological Society
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/44/1/ranga2011.pdf
Sridhar Kumar, Ch and Ranga Rao, G V and Sireesha, K and Kumar, P L (2011) Isolation and Characterization of Baculoviruses from Three Major Lepidopteran Pests in the Semi-Arid Tropics of India. Indian Journal of Virology, 22 (1). pp. 29-36. ISSN 0970-2822