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Characterization of AhMITE1 transposition and its association with the mutational and evolutionary origin of botanical types in peanut (Arachis spp.)

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Relation http://oar.icrisat.org/60/
http://dx.doi.org/10.1007/s00606-010-0373-3
 
Title Characterization of AhMITE1 transposition and its association with the mutational and
evolutionary origin of botanical types in peanut (Arachis spp.)
 
Creator Gowda, M V C
Bhat, R S
Sujay, V
Kusuma, P
Kumari, Varsha
Varshney, R K
Bhat, S
 
Subject Groundnut
 
Description AhMITE1 is an active miniature inverted
repeat transposable element (MITE) in peanut (Arachis
hypogaea L). Its transpositional activity from a particular
(FST1-linked) site within the peanut genome was checked
using AhMITE1-specifc PCR, which used a forward primer
annealing to the 50-flanking sequence and a reverse primer
binding to AhMITE1. It was found that transposition activation
was induced by stresses such as ethyl methane
sulfonate (EMS), gamma irradiation, environmental conditions,
and tissue culture. Excision and insertion of
AhMITE1 at this particular site among the mutants led to
gross morphological changes resembling alternate subspecies
or botanical types. Analysis of South American landraces
revealed the presence of AhMITE1 at the site among
most of the spp. fastigiata types, whereas the element was
predominantly missing from spp. hypogaea types, indicating
its strong association. Four accessions of the primitive
allotetraploid, A. monticola were devoid of AhMITE1
at the site, indicating only recent activation of the element,
possibly because of the ‘‘genomic shock’’ resulting from
hybridization followed by allopolyploidization.
 
Publisher Springer
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
application/pdf
 
Language en
en
 
Rights —
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Identifier http://oar.icrisat.org/60/1/1311_merged.pdf
http://oar.icrisat.org/60/2/1311_published.pdf
Gowda, M V C and Bhat, R S and Sujay, V and Kusuma, P and Kumari, Varsha and Varshney, R K and Bhat, S (2011) Characterization of AhMITE1 transposition and its association with the mutational and evolutionary origin of botanical types in peanut (Arachis spp.). Plant Systematics and Evolution, 291 (3-4). pp. 153-158.