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Analysis of Genetic Diversity in Pongamia [Pongamia pinnata (L)Pierrre] using AFLP Markers

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Relation http://oar.icrisat.org/69/
 
Title Analysis of Genetic Diversity in Pongamia [Pongamia pinnata (L)Pierrre] using AFLP Markers
 
Creator Thudi, M
Manthena, R
Wani, S P
Tatikonda, L
Hoisington, D A
Varshney, R K
 
Subject Oil seeds
 
Description In recent years, Pongamia has been considered as important renewable source of biodiesel, however not much molecular
information is available in this species. Molecular characterization of this legume tree will enhance our understanding in
improving the optimal yields of oil through breeding and enable us to meet the future demands for biodiesel. To assess the
molecular genetic diversity in 48 Pongamia pinnata accessions collected from six different states of India, amplified fragment
length polymorphism (AFLP) marker system was employed. Five AFLP primer combinations produced 520 discernible
fragments, of which 502 (96.5%) were polymorphic. AFLP primer informativeness was estimated evaluating four parameters
namely polymorphism information content (PIC), effective multiplex ratio (EMR), marker index (MI) and resolving power (RP).
In total, 51 unique fragments were detected of which 19 unique fragments were observed with primer combination E-ACG /
M-CTA. Although neighbour joining (NJ) method did not group accessions strictly according to their region of collection, a
good level of genetic diversity was observed in examined germplasm. However, accessions collected from Karnataka showed
comparatively higher diversity than accessions from other states. The diverse accessions identified in this study may be
useful in Pongamia pinnata improvement to meet the future demands of biodiesel.
 
Publisher Springer
 
Date 2010
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/69/1/thudi1.pdf
Thudi, M and Manthena, R and Wani, S P and Tatikonda, L and Hoisington, D A and Varshney, R K (2010) Analysis of Genetic Diversity in Pongamia [Pongamia pinnata (L)Pierrre] using AFLP Markers. Journal of Plant Biochemistry and Biotechnlogy, 19 (2). pp. 209-216.