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A QTL study on late leaf spot and rust revealed one major QTL for molecular breeding for rust resistance in groundnut Arachis hypogaea L.)

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Relation http://oar.icrisat.org/74/
http://dx.doi.org/10.1007/s00122-010-1366-x
 
Title A QTL study on late leaf spot and rust revealed one major QTL for molecular breeding for rust resistance in groundnut Arachis hypogaea L.)
 
Creator Khedikar, Y P
Gowda, M V C
Sarvamangala, C
Patgar, K V
Upadhyaya, H D
Varshney, R K
 
Subject Groundnut
 
Description Late leaf spot (LLS) and rust are two major
foliar diseases of groundnut (Arachis hypogaea L.) that
often occur together leading to 50–70% yield loss in the
crop. A total of 268 recombinant inbred lines of a mapping
population TAG 24 £ GPBD 4 segregating for LLS and
rust were used to undertake quantitative trait locus (QTL)
analysis. Phenotyping of the population was carried out
under artiWcial disease epiphytotics. Positive correlations
between diVerent stages, high to very high heritability and
independent nature of inheritance between both the
diseases were observed. Parental genotypes were screened
with 1,089 simple sequence repeat (SSR) markers, of which
67 (6.15%) were found polymorphic. Segregation data
obtained for these markers facilitated development of partial
linkage map (14 linkage groups) with 56 SSR loci.
Composite interval mapping (CIM) undertaken on genotyping
and phenotyping data yielded 11 QTLs for LLS (explaining
1.70–6.50% phenotypic variation) in three environments
and 12 QTLs for rust (explaining 1.70–55.20% phenotypic
variation). Interestingly a major QTL associated with rust
(QTLrust01), contributing 6.90–55.20% variation, was identiWed
by both CIM and single marker analysis (SMA). A
candidate SSR marker (IPAHM 103) linked with this QTL
was validated using a wide range of resistant/susceptible
breeding lines as well as progeny lines of another mapping
population (TG 26 £ GPBD 4). Therefore, this marker
should be useful for introgressing the major QTL for rust in
desired lines/varieties of groundnut through markerassisted
backcrossing.
 
Publisher Springer Verlag
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/74/1/rkv20103.pdf
Khedikar, Y P and Gowda, M V C and Sarvamangala, C and Patgar, K V and Upadhyaya, H D and Varshney, R K (2011) A QTL study on late leaf spot and rust revealed one major QTL for molecular breeding for rust resistance in groundnut Arachis hypogaea L.). TAG Theoretical and Applied Genetics, 121 (5). pp. 971-984.