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Characterisation of pathogenic and molecular diversity in Sclerospora graminicola, the causal agent of pearl millet downy mildew

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Relation http://oar.icrisat.org/84/
http://dx.doi.org/10.1080/03235400801939896
 
Title Characterisation of pathogenic and molecular diversity in Sclerospora graminicola, the causal agent of pearl millet downy mildew
 
Creator Sharma, R
Rao, V P
Varshney, R K
Prasanth, V P
Kannan, S
Thakur, R P
 
Subject Millets
 
Description Genotypic diversity among 46 isolates of Sclerospora graminicola collected from seven
states in India during 1992–2005 was determined through pathotyping and AFLP
analysis. A high level of variation was observed among the isolates for downy mildew
incidence, latent period and virulence index. Based on the reaction on a set of nine pearl
millet lines, 46 isolates were classified in 21 pathotypes. Quantitative differences in
virulence levels of the test isolates were assessed by calculating the virulence index
(disease incidence 6 latent period 7 1). A dendrogram generated by the average linkage
cluster analysis of virulence index clustered the 46 isolates into eight groups. Regionspecific
grouping of five isolates from Gujarat and six from Rajasthan was observed
within two distinct groups. Temporal variation was also observed among the isolates
collected from the same location and same host over the years. A total of 297 bands
were scored following selective amplification with three primer combinations E-TT/MCAG,
E-AT/M-CAG and E-TG/M-CAT and all of them were polymorphic. Cluster
analysis of AFLP data clustered the test isolates into seven groups. Analysis of
molecular variance indicated that variation in the S. graminicola populations was largely
due to differences among the isolates within the states.
 
Publisher Taylor & Francis
 
Date 2010
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/84/1/ArchOfPhytoPlProt43_6_538-551_2010.pdf
Sharma, R and Rao, V P and Varshney, R K and Prasanth, V P and Kannan, S and Thakur, R P (2010) Characterisation of pathogenic and molecular diversity in Sclerospora graminicola, the causal agent of pearl millet downy mildew. Archives of Phytopathology and Plant Protection, 43 (6). pp. 538-551. ISSN 0323-5408