Record Details

Genetic mapping and quantitative trait locus analysis of resistance to sterility mosaic disease in pigeonpea [Cajanus cajan (L.) Millsp.]

OAR@ICRISAT

View Archive Info
 
 
Field Value
 
Relation http://oar.icrisat.org/94/
http://dx.doi.org/10.1016/j.fcr.2011.04.011
 
Title Genetic mapping and quantitative trait locus analysis of resistance to sterility
mosaic disease in pigeonpea [Cajanus cajan (L.) Millsp.]
 
Creator Gnanesh, B N
Bohra, A
Sharma, M
Byregowda, M
Pandey, S
Wesley, V
Saxena, R K
Saxena, K B
Kavi Kishor, P B
Varshney, R K
 
Subject Pigeonpea
 
Description Sterility mosaic disease (SMD), considered as the “green plague of pigeonpea” and caused by pigeonpea
sterility mosaic virus (PPSMV) is one of the major biotic factors, which leads to heavy yield losses
and hence poses a big challenge for pigeonpea production in the Indian subcontinent. Variability in the
sterility mosaic pathogen revealed the occurrence of five different isolates in India. Among them, three
distinct SMD isolates have been characterized, viz., Patancheru, Bangalore and Coimbatore. Molecular
tools offer a viable option to tackle these biotic stresses via identification of the genomic regions associated
with the trait such as SMD resistance. With an aim of identifying the gene(s)/QTLs linked with SMD
resistance, two F2 populations, i.e. ICP 8863×ICPL 20097 (segregating for Patancheru SMD isolate) and
TTB 7×ICP 7035 (segregating for both Patancheru and Bangalore SMD isolates) were developed and F2:3
families were phenotyped for resistance to respective isolate(s) of SMD. After screening over 3000 SSR
markers on parental genotypes of each mapping population, intra-specific genetic maps comprising of
11 linkage groups and 120 and 78 SSR loci were developed for ICP 8863×ICPL 20097 and TTB 7×ICP
7035 populations, respectively. Composite interval mapping (CIM) based QTL analysis by using genetic
mapping and phenotyping data provided four QTLs for Patancheru SMD isolate and two QTLs for Bangalore
SMD isolate. Identification of different QTLs for resistance to Patancheru and Bangalore SMD isolates
is an indication of involvement of different genes conferring the resistance to these two SMD isolates.
One QTL namely qSMD4 identified within an interval of 2.8cM on LG 7 explaining 24.72% of phenotypic
variance, once it is validated in other genetic background, seems to be a promising QTL for use in marker
assisted selection. In summary, this is the first study on development of intra-specific genetic maps and
identification of QTLs for SMD resistance in pigeonpea
 
Publisher Elsevier
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/94/1/FIELD5470.pdf
Gnanesh, B N and Bohra, A and Sharma, M and Byregowda, M and Pandey, S and Wesley, V and Saxena, R K and Saxena, K B and Kavi Kishor, P B and Varshney, R K (2011) Genetic mapping and quantitative trait locus analysis of resistance to sterility mosaic disease in pigeonpea [Cajanus cajan (L.) Millsp.]. Field Crops Research, 123. pp. 53-61.