Record Details

Ensuring Genetic Purity of Pigeonpea Hybrids by Incorporating a Naked-Eye Polymorphic Marker in A and B Lines

OAR@ICRISAT

View Archive Info
 
 
Field Value
 
Relation http://oar.icrisat.org/109/
http://dx.doi.org/10.2135/cropsci2010.11.0655
 
Title Ensuring Genetic Purity of Pigeonpea Hybrids
by Incorporating a Naked-Eye Polymorphic
Marker in A and B Lines
 
Creator Saxena, K B
Vales, M I
Kumar, R V
Sultana, R
Srivastava, R K
 
Subject Pigeonpea
 
Description To enhance the productivity of pigeonpea [Cajanus
cajan (L.) Millspaugh] a hybrid breeding
technology, based on the cytoplasmic nuclear
male-sterility (CMS) system and partial natural
outcrossing, is currently been used. However,
there are diffi culties to maintain genetic purity
of the hybrids and their parents. The incorporation
of an easily identifi able morphological
marker (naked eye polymorphism [NEP]) could
be used to determine seed purity. The morphological
marker selected for this study, obcordate
leaf, is not present in cultivated pigeonpea; it is
inherited as a single recessive gene and can be
observed soon after planting (approx. 6 wk). To
incorporate the obcordate leaf shape into hybrid
parents, the trait was transferred from the germplasm
accession ICP 5529 into male-sterile (A
lines) and the corresponding maintainers (B
lines). The hybrids derived from crosses involving
obcordate leaf A lines and normal leaf fertility
restorers (R lines) were fully fertile and had
normal lanceolate leaves; thus the difference
between A line and hybrids was clear. The use
of obcordate leaf as a NEP marker in pigeonpea
would contribute to preserve parental line purity
and confi rm hybrid status.
 
Publisher Crop Science Society of America
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/109/1/isabel.pdf
Saxena, K B and Vales, M I and Kumar, R V and Sultana, R and Srivastava, R K (2011) Ensuring Genetic Purity of Pigeonpea Hybrids by Incorporating a Naked-Eye Polymorphic Marker in A and B Lines. Crop Science, 51 (4). pp. 1564-1570.