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Generation of Expressed Sequence Tags and Marker Development for Sterility Mosaic Disease Resistance in Pigeonpea

OAR@ICRISAT

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Relation http://oar.icrisat.org/130/
 
Title Generation of Expressed Sequence Tags and
Marker Development for Sterility Mosaic Disease Resistance in Pigeonpea
 
Creator Gnanesh, B N
 
Subject Chickpea
 
Description Sterility mosaic disease (SMD), an important biotic constraint on pigeonpea (Cajanus cajan (L) Millsp.) in the Indian subcontinent, is caused by Pigeonpea sterility mosaic virus and transmitted by the eriophyid mite. Investigations were carried out to develop F2:3 mapping population involving resistant (ICP 7035) and susceptible (TTB 7) parents, development of EST SSR markers, construction of linkage map and identification of QTLs for SMD resistance. F1s of the susceptible × resistant cross were susceptible indicating susceptibility to be dominant over resistance. The pattern of frequency distribution of SMD incidence in the F2:3 was found to be continuous depicting quantitative nature of resistance. Totally 3,788 high quality ESTs were generated from SMD challenged genotypes of ICP 7035 and TTB 7. Sequence clustering and assembly process of all the assembled 3,788 ESTs resulted in 1,308 unigenes. Out of 3320 SSR markers (3236 genomic+84 genic) screened for SMD in TTB 7 and ICP 7035, 2107 (63.5%) could amplify and 84 (83 genomic + 01 genic) SSR markers were found to be polymorphic (2.5%). A total of 82 markers were mapped on 11 linkage groups (LGs) of pigeonpea spanning 539.5 cM and two markers remained ungrouped. Number of markers mapped per linkage group ranged from three (LG 11) to twelve (LG 7). The present study yielded two QTLs for Bengaluru isolate of SMD positioned on LG 3 and LG 7 accounting 10.39 per cent and 15.74 of the phenotypic variation respectively. For Patancheru isolate also, two QTLs were identified and the first QTL explained 12.3 per cent phenotypic variation and the second QTL explained 24.69 per cent of phenotypic variation.
 
Date 2010
 
Type Thesis
NonPeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/130/1/merged_document-4.pdf
Gnanesh, B N (2010) Generation of Expressed Sequence Tags and Marker Development for Sterility Mosaic Disease Resistance in Pigeonpea. PhD thesis, University of Agricultural Sciences, Bangalore.