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EST-SSR based estimates on functional genetic variation in a barley (Hordeum vulgare L.) collection from Egypt

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Relation http://oar.icrisat.org/229/
http://dx.doi.org/10.1007/s10722-009-9489-0
 
Title EST-SSR based estimates on functional genetic variation in a barley (Hordeum vulgare L.) collection from Egypt
 
Creator Salem, K F M
Varshney, R K
Roder, M S
Borner, A
 
Subject Agriculture-Farming, Production, Technology, Economics
 
Description Genetic diversity was investigated in a set of 27 barley genotypes, originated from Egypt, using 23 expressed sequence tag (EST)-derived simple sequence repeats (eSSRs), representatives of the seven barley chromosomes. Ninety-five alleles were detected among all the barley accessions. For 22 polymorphic eSSRs, the number of alleles per locus varied from 2 to 12, with a mean of 4.318 alleles per locus. The lowest and the largest number of alleles per locus among the
seven homeologous groups was observed in homeologous group 5H and 4H with 3.00 and 6.33, respectively. The gene diversity increased as the number of alleles increased. Gene diversity for 22 ESTs loci varied from 0.137 for GBM1404 to 0.896 for GBM1015 with an average of 0.563. A significant
correlation coefficient between gene diversity and the number of alleles was high, r = 0.741 (P\0.01). Cluster analysis was conducted based on eSSRs data to group the barley genotypes and to construct a dendrogram. Four groups can be distinguished by truncating the dendrogram at gs value of 0.77.
 
Publisher Kluwer
 
Date 2010
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/229/1/n67.pdf
Salem, K F M and Varshney, R K and Roder, M S and Borner, A (2010) EST-SSR based estimates on functional genetic variation in a barley (Hordeum vulgare L.) collection from Egypt. Genetic Resources and Crop Evolution, 57. pp. 515-525.