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Cloning, phylogeny and three dimensional structure analysis of the gene encoding Interferon-ε of Sheep (Ovis aries)

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Title Cloning, phylogeny and three dimensional structure analysis of the gene encoding Interferon-ε of Sheep (Ovis aries)
 
Creator Nagarajan, Govindasamy
Kanagarajadurai, Karuppiah
Meena, Amar Singh
Kumar, Rajiv
Sharma, Priya Rajkumar
Babu, KS Dinesh
Premnath, D
Thirumaran, SMK
Pachaiyappan, Kuppachari
 
Subject Bioinformatic analysis
Interferon-ε
Liver
Sheep
Three dimensional structure
 
Description 232-240
Interferon (IFN) epsilon (ε) has been identified as an important protein of interest, which plays a major role in innate
immune system of mammals. Indian Avishaan sheep is well known for its twinning and triplet traits. Hence, the present study
has been undertaken with the aim to characterize Indian Avishaan sheep IFNε, although reports found on Ovis aries
IFN-epsilon and Arabian Camel IFN-epsilon. The cDNA of type I interferon viz., Interferon-ε from the liver of Avishaan sheep
has been amplified by PCR and subsequently cloned for sequence analysis. Sequence analysis revealed that Avishaan sheep
shared 100 % both at nucleotide and amino acid levels with that of Rambouillet sheep. With other artiodactyls, the range of
identity of Avishhan sheep was 85- 99 % and 78- 98 %, at nucleotide and amino acid levels, respectively. Perissodactyls shared
84-85 % and 77-78 % identity at nucleotide and amino acid levels, respectively, with that of Avishaan sheep from India. Three
dimensional structure of Interferon-ε protein of Avishaan sheep was also discussed. Phylogenetic analysis based on amino acid
sequences indicated the close relationship in Interferon-ε gene between sheep and other artiodactyls. The structural and
functional specific residues noticed across amino acid sequences shows specificity towards orthologs, which needs further
characterization.
 
Date 2024-03-06T08:41:53Z
2024-03-06T08:41:53Z
2024-03
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/63531
https://doi.org/10.56042/ijbb.v61i4.1230
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJBB Vol.61(04) [April 2024]