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Multi-locus genome-wide association studies (ML-GWAS) reveal novel genomic regions associated with seedling and adult plant stage leaf rust resistance in bread wheat (Triticum aestivum L.)

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Relation http://oar.icrisat.org/12571/
https://www.nature.com/articles/s41437-022-00525-1
https://doi.org/10.1038/s41437-022-00525-1
 
Title Multi-locus genome-wide association studies (ML-GWAS) reveal novel genomic regions associated with seedling and adult plant stage leaf rust resistance in bread wheat (Triticum aestivum L.)
 
Creator Vikas, V K
Pradhan, A K
Budhlakoti, N
Mishra, D C
Chandra, T
Bhardwaj, S C
Kumar, S
Sivasamy, M
Jayaprakash, P
Nisha, R
Shajitha, P
Peter, J
Geetha, M
Mir, R R
Singh, K
Kumar, S
 
Subject Wheat
 
Description Leaf rust is one of the important diseases limiting global wheat production and productivity. To identify quantitative trait nucleotides (QTNs) or genomic regions associated with seedling and adult plant leaf rust resistance, multilocus genome-wide association studies (ML-GWAS) were performed on a panel of 400 diverse wheat genotypes using 35 K single-nucleotide polymorphism (SNP) genotyping assays and trait data of leaf rust resistance. Association analyses using six multi-locus GWAS models revealed a set of 201 significantly associated QTNs for seedling and 65 QTNs for adult plant resistance (APR), explaining 1.98–31.72% of the phenotypic variation for leaf rust. Among these QTNs, 51 reliable QTNs for seedling and 15 QTNs for APR were consistently detected in at least two GWAS models and were considered reliable QTNs. Three genomic regions were pleiotropic, each controlling two to three pathotype-specific seedling resistances to leaf rust. We also identified candidate genes, such as leucine-rich repeat receptor-like (LRR) protein kinases, P-loop containing nucleoside triphosphate hydrolase and serine-threonine/tyrosine-protein kinases (STPK), which have a role in pathogen recognition and disease resistance linked to the significantly associated genomic regions. The QTNs identified in this study can prove useful in wheat molecular breeding programs aimed at enhancing resistance to leaf rust and developing next-generation leaf rust-resistant varieties.
 
Publisher Springer Nature
 
Date 2022-04-13
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights cc_by
 
Identifier http://oar.icrisat.org/12571/1/Heredity_128_434-449_2022.pdf
Vikas, V K and Pradhan, A K and Budhlakoti, N and Mishra, D C and Chandra, T and Bhardwaj, S C and Kumar, S and Sivasamy, M and Jayaprakash, P and Nisha, R and Shajitha, P and Peter, J and Geetha, M and Mir, R R and Singh, K and Kumar, S (2022) Multi-locus genome-wide association studies (ML-GWAS) reveal novel genomic regions associated with seedling and adult plant stage leaf rust resistance in bread wheat (Triticum aestivum L.). Heredity, 128. pp. 434-449. ISSN 1365-2540