Record Details

In Silico and In Vitro Investigation of Phytochemicals Against Shrimp AHPND Syndrome Causing PirA,B Toxins of Vibrio parahaemolyticus

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title In Silico and In Vitro Investigation of Phytochemicals Against Shrimp AHPND Syndrome Causing PirA,B Toxins of Vibrio parahaemolyticus
Not Available
 
Creator Jahangir Ahmed
Irfan Navabshan
Sneha Unnikrishnan
Logesh Radhakrishnan
K.P. Kumaraguru Vasagam
Karthikeyan Ramalingam
 
Subject Shrimps disease
Acute hepatopancreatic necrosis diseases (AHPND)
Vibrio parahaemolyticus
PirA,B toxins
Phytocompounds
Molecular docking
Molecular dynamics
 
Description Not Available
In Southeast Asia, the penaeid shrimp aquaculture production faces a new pandemic bacte
rial disease called acute hepatopancreatic necrosis disease (AHPND). The highly profit
able pacific white shrimp, Litopenaeus vannamei, has become a challenging species due
to severe lethal infections. Recent research has identified a critical pathogen, Vibrio para
haemolyticus, which caused significant loss in the shrimp industry. The disease pathway
involves a virulence plasmid encoding binary protein toxins (PirA/B) that cause cell death.
The protein toxins were inherited and conjugatively transferred to other Vibrio species
through a post-segregational killing system. In this study, “in silico” (Glide, 2021) analy
sis identified four phytocompounds as myricetin (Myr), ( +)-taxifolin (TF), (-)-epigallocat
echin gallate (EGCG), and strychnine (STN) which could be most effective against both
the toxins concerning its docking score and affinity. The interactions of complexes and the
critical amino acids involved in docking were analyzed using the Discovery Studio (ver
sion 2016). Molecular dynamic studies showed lower root mean square deviations (RMSD)
and improved stabilization of ( +)-taxifolin (TF) and (-)-epigallocatechin-3-gallate (EGCG)
against both the protein toxins. The antibacterial potential of all four selected compounds
had tested against pathogenic strains of V. parahaemolyticus through minimum inhibi
tory concentration (MIC) and minimum bactericidal concentration (MBC). The best MBC
results were observed at concentrations of 1 mg/mL for EGCG and 1.25 mg/mL for TF.
Moreover, the complete reduction of viable cell counts in the in vitro bactericidal activity
had recorded after 24 h of incubation
Not Available
 
Date 2024-03-14T11:00:51Z
2024-03-14T11:00:51Z
2023-03-29
 
Type Journal
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/81644
 
Language English
 
Relation Not Available;
 
Publisher Not Available