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Pathogenic potential of biofilm-producing methicillin-resistant Staphylococcus aureus in BALB/c mice

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Title Pathogenic potential of biofilm-producing methicillin-resistant Staphylococcus aureus in BALB/c mice
 
Creator Sannat, Chandrahas
Hirpurkar, SD
Shakya, Sanjay
Jolhe, Nidhi Rawat DK
Singh, Jasmeet
Jain, Tripti
Gumasta, Poornima
Patyal, Anil
Tripathi, SM
Kalim, MO
Barwa, Deepti Kiran
Deshmukh, Shivesh Kumar
 
Subject Cow milk
Disease activity score (DAS)
Mastitic milk
MRSA pathogenesis
Multidrug resistance (MDR)
 
Description 206-213
Biofilm-forming methicillin-resistant Staphylococcus aureus (MRSA) is an emerging pathogen that adversely affects
animal and human health. World Health Organization (WHO) has designated MRSA as a high-priority pathogen for
research and development. In this study, we have investigated the pathogenic potential of MRSA recovered from mastitic
milk of cow. The MRSA was initially characterized for coagulase, haemolytic and DNase activity followed by its biofilm
forming ability. Further, an intravenous murine model of MRSA was developed using multiparameter approach comprising
of disease activity score, viable bacterial count in blood and tissues; and, detection of biofilm mass in tissue. Infection was
successfully established in mice following intravenous inoculation of 3 × 108 colony forming unit (CFU) per mL of MRSA.
Fifty percent of MRSA-challenged mice died after infection whereas mice survived exhibited disease activity score >25.
Significantly higher MRSA count was recorded in blood, liver and kidney of MRSA-challenged mice as compared to
healthy mice (P
 
Date 2024-03-15T09:27:14Z
2024-03-15T09:27:14Z
2024-03
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/63580
https://doi.org/10.56042/ijeb.v62i03.3410
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJEB Vol.62(03) [March 2024]