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Molecular profiling of BADH2 locus reveals distinct functional allelic polymorphism associated with fragrance variation in Indian aromatic rice germplasm

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Relation http://oar.icrisat.org/12589/
https://link.springer.com/article/10.1007/s12298-022-01181-9
https://doi.org/10.1007/s12298-022-01181-9
 
Title Molecular profiling of BADH2 locus reveals distinct functional allelic polymorphism associated with fragrance variation in Indian aromatic rice germplasm
 
Creator Singh, G
Krishnan, S G
Kumar, A
Vinod, K K
Bollinedi, H
Ellur, R K
Nagarajan, M
Bhowmick, P K
Madhav, S M
Singh, K
Singh, A K
 
Subject Rice
 
Description Allelic variability of the aroma gene, betaine aldehyde dehydrogenase 2 (BADH2) was studied in a random subset of indigenous aromatic rice germplasm along with a few exotic aromatic accessions. Use of functional markers of four badh2 alleles identified that the test panel possessed only two alleles, badh2-E7 and badh2-p-5′UTR. Two other alleles, badh2.2 and badh2-E4-5.2 were absent. Based on the alleles present, four functional polymorphisms (FP) were detected, namely FP1 to FP4. 188 genotypes possessed FP1 having both the badh2-p-5′UTR and badh2-E7 (71.8%) alleles. The badh2 allele with FP1 is named badh2-E7-p. 39 genotypes (14.9%) possessed only the badh2-p-5′UTR allele (FP3), while three genotypes were found to carry only the badh2-E7 allele (FP2). We also found that 32 genotypes (12.2%) did not have any of the target aroma alleles tested in this study (FP4). Interestingly, for badh2-p-5′UTR marker, the expected 198 bp amplicon for the non-aromatic allele could not be detected among any of the genotypes tested. Instead, an amplicon of 456 bp length appeared with 100% presence in the non-aromatic checks. Notwithstanding, the 456 bp allele also showed a 16% presence among the aromatic lines. This article forms the first report of this allele, named badh2-p1, among aromatic rice. Quantification of 2-Acetyl-1-Pyrroline (2AP) content and sensory evaluation among the test genotypes showed that those with FP1 are highly aromatic than the genotypes carrying other types of FPs. But, a few strongly aromatic lines showed lower 2AP content. The BADH2 characterization carried out in this study is suggestive of identifying the additional gene(s)/ allele(s) governing aroma among the Indian fragrant rice.
 
Publisher Springer
 
Date 2022-05-10
 
Type Article
PeerReviewed
 
Identifier Singh, G and Krishnan, S G and Kumar, A and Vinod, K K and Bollinedi, H and Ellur, R K and Nagarajan, M and Bhowmick, P K and Madhav, S M and Singh, K and Singh, A K (2022) Molecular profiling of BADH2 locus reveals distinct functional allelic polymorphism associated with fragrance variation in Indian aromatic rice germplasm. Physiology and Molecular Biology of Plants (TSI), 28. pp. 1013-1027. ISSN 0971-5894