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In vitro validation of anti-diabetic and free radical scavenging property of Naaval Kottai Mathirai, a promising Siddha drug for the management of Diabetes mellitus

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Title In vitro validation of anti-diabetic and free radical scavenging property of Naaval Kottai Mathirai, a promising Siddha drug for the management of Diabetes mellitus
 
Creator S, Sivakkumar
L, Juliet
R, Meenakumari
 
Subject Anti-diabetic assay
Anti-oxidant assay
Naval Kottai Mathirai
Siddha medicine
 
Description 291-296
The World Health Organization (WHO) reported 422 million total Diabetic population worldwide and about 1.5 million death rates caused due to it. Diabetes Mellitus (Madhumegam) is a disease classified under Neerinai Perkukkal Noi (diseases causing excessive urination) caused due to increased kapam humour. Naaval Kottai Mathirai (NKM) is a unique herbal Siddha drug prepared from Aristolochia bracteolata which has bitter taste and Syzygium cumini which has astringent taste. The herbal formulation NKM neutralizes kapam and hence effective in Madhumegam. In vitro studies like alpha-amylase, alpha-glucosidase inhibitory assays were performed to prove the anti diabetic effect of the drug NKM. DPPH (2,2- Diphenyl 1-2 picrylhydrazyl) assay, hydrogen peroxide scavenging assay (H₂O₂) and nitric oxide radical scavenging assay were doneto evaluate its antioxidant potency. The results showed that minimum alpha amylase inhibitory activity ranged from19.42±5.92 to 60.92±3.98%, with an IC50 value 70.9±12.37 μg/mL, when it is compared with standard drug acarbose, whichshowed inhibition ranged from 39.83±2.18 to 91.08±5.43 with an IC50 value 13.29±1.95 μg/mL in various concentrationlevels of NKM. The IC50 value of Butylated Hydroxyanisole (BHA) was noted as 48.61±1.69 μg/mL and NKM as67.19±7.81 μg/mL for the H2O2 scavenging antioxidant activity. In nitrogen scavenging antioxidant activity, it was observedthat IC50 value of gallic acid was 23.02 μg/mL and NKM was 79.13 μg/mL. In DPPH scavenging activity, for ascorbic acid,the standard showed its IC50 value as 13.36 μg/mL and NKM as 53.39 μg/mL. The phytoconstituents present in NKM mayenrich the antidiabetic and antioxidant properties of the drug.
 
Date 2024-03-19T11:32:53Z
2024-03-19T11:32:53Z
2024-03
 
Type Article
 
Identifier 0975-1068 (Online); 0972-5938 (Print)
http://nopr.niscpr.res.in/handle/123456789/63600
https://doi.org/10.56042/ijtk.v23i3.1458
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJTK Vol.23(3) [March 2024]