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Development and validation of a novel core set of KASP markers for the traits improving grain yield and adaptability of rice under direct-seeded cultivation conditions

OAR@ICRISAT

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Relation http://oar.icrisat.org/12593/
https://www.sciencedirect.com/science/article/pii/S0888754322000143
https://doi.org/10.1016/j.ygeno.2022.110269
 
Title Development and validation of a novel core set of KASP markers for the traits improving grain yield and adaptability of rice under direct-seeded cultivation conditions
 
Creator Sandhu, N
Singh, J
Singh, G
Sethi, M
Singh, M P
Pruthi, G
Raigar, O P
Kaur, R
Kaur, R
Sarao, P S
Lore, J S
Singh, U M
Dixit, S
Sagare, D B
Singh, S P
Satturu, V
Singh, V K
Kumar, A
 
Subject Abiotic Stress
Biotic Stress
Rice
Genetics and Genomics
 
Description The development and utilization of molecular-markers play an important role in genomics-assisted breeding during pyramiding of valuable genes. The aim of present study was to develop and validate a novel core-set of KASP (Kompetitive Allele-Specific PCR) markers associated with traits improving rice grain yield and adaptability under direct-seeded cultivation conditions. The 110 phenotypically validated KASP assays out of 171 designed KASP, include assays for biotic-resistance genes, anaerobic germination, root-traits, grain yield, lodging resistance and early-uniform emergence. The KASP assays were validated for their robustness and reliability at five different levels using diverse germplasm, segregating and advanced population, comparison with SSR markers and on F1s. The present research work will provide (i) breeding material in form of anticipated pre-direct-seeded adapted rice varieties (ii) single improved breeding line with many useful genes and (iii) KASP assay information for the useful QTL/genes providing grain yield and adaptability to rice under direct-seeded cultivation conditions.
 
Publisher Elsevier
 
Date 2022-01-19
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights cc_by_nc_nd
 
Identifier http://oar.icrisat.org/12593/1/Genomics_114_1-18_2022.pdf
Sandhu, N and Singh, J and Singh, G and Sethi, M and Singh, M P and Pruthi, G and Raigar, O P and Kaur, R and Kaur, R and Sarao, P S and Lore, J S and Singh, U M and Dixit, S and Sagare, D B and Singh, S P and Satturu, V and Singh, V K and Kumar, A (2022) Development and validation of a novel core set of KASP markers for the traits improving grain yield and adaptability of rice under direct-seeded cultivation conditions. Genomics, 114. pp. 1-18. ISSN 0888-7543