Biocontrol potentials of novel indigenous Trichoderma isolates against Fusarium wilt of chickpea
OAR@ICRISAT
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Relation |
http://oar.icrisat.org/12596/
https://link.springer.com/article/10.1007/s42360-021-00442-z https://doi.org/10.1007/s42360-021-00442-z |
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Title |
Biocontrol potentials of novel indigenous Trichoderma isolates against Fusarium wilt of chickpea
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Creator |
Ramanagouda, G
Naik, M K Sharma, M |
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Subject |
Chickpea
Plant Pathology |
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Description |
Forty Trichoderma isolates were collected from North-eastern dry zone of Karnataka to assess abiotic and biotic stress tolerance and chickpea Fusarium wilt management abilities. Among the isolates collected, Trichoderma asperellum and Hypocrea lixii species were predominantly distributed in the study area. In-vitro experiments showed that isolate T-46, T-43, T-17, T-13, T-36 and T-45 are effective in antagonistic and volatile compounds (VCs) production against chickpea pathogens. The NaCl salt range from 400 to 600 mM and temperature from 20 to 35 °C was optimum for normal growth and sporulation of Trichoderma isolates. The salt concentration above 600 mM NaCl and temperature 37 °C was found detrimental to Trichoderma mycelial growth, conidia and chlamydospores productions. Based on the abiotic and biotic stress tolerance five isolates (T-10, T-17, T-25, T-46, T-50) were selected for field experiment to manage chickpea Fusarium wilt. Treatment with T-17 and T-46 showed highest wilt inhibition over control in Annigeri cultivar in the sick soil condition. Under glasshouse conditions isolate T-46 exhibited delayed onset of Fusarium wilt incidence in JG 62 cultivar. The lowest AUDPC and relative AUDPC was observed in T-46 (70.50 ± 1.00 and 2.43 ± 0.03) and carbendazim (73.44 ± 4.12 and 2.53 ± 0.14) treated treatments. This finding provides insights on indigenous Trichoderma isolates tolerant to abiotic and biotic stress and can be used to alleviate chickpea Fusarium wilt in farmer fields.
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Publisher |
Springer
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Date |
2021-11-17
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Type |
Article
PeerReviewed |
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Identifier |
Ramanagouda, G and Naik, M K and Sharma, M (2021) Biocontrol potentials of novel indigenous Trichoderma isolates against Fusarium wilt of chickpea. Indian Phytopathology, 75. pp. 135-146. ISSN 2248-9800
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