Record Details

Discovery of Major Quantitative Trait Loci and Candidate Genes for Fresh Seed Dormancy in Groundnut

OAR@ICRISAT

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Field Value
 
Relation http://oar.icrisat.org/12598/
https://www.mdpi.com/2073-4395/12/2/404
https://doi.org/10.3390/agronomy12020404
 
Title Discovery of Major Quantitative Trait Loci and Candidate Genes for Fresh Seed Dormancy in Groundnut
 
Creator Bomireddy, D
Gangurde, S S
Variath, M T
Janila, P
Manohar, S S
Sharma, V
Parmar, S
Deshmukh, D
Reddisekhar, M
Reddy, D M
Sudhakar, P
Reddy, B V B
Varshney, R K
Guo, B
Pandey, M K
 
Subject Groundnut
Genetics and Genomics
 
Description Spanish bunch groundnut varieties occupy most of the cultivated area in Asia and Africa, and these varieties lack required 2-3 weeks of fresh seed dormancy (FSD) hampering kernel quality. Genomic breeding can help to improve commercial groundnut cultivars for FSD in a shorter time with greater precision. In this regard, a recombinant inbred line (RIL) population from the cross ICGV 02266 (non-dormant) × ICGV 97045 (dormant) was developed and genotyped with a 5 K mid-density genotyping assay. A linkage map was constructed with 325 SNP loci spanning a total map length of 2335.3 cM and five major QTLs were identified on chromosomes Ah01, Ah11, Ah06, Ah16 and Ah17. Based on differential gene expression using transcriptomic information from dormant (Tifrunner) and non-dormant (ICGV 91114) genotypes, histone deacetylases, histone-lysine N-methyltransferase, cytochrome P450, protein kinases, and ethylene-responsive transcription factor were identified as key regulators involved in the hormonal regulation of dormancy. Six Kompetitive Allele Specific PCR (KASP) markers were successfully validated in the diverse panel including selected RILs of the same population and germplasm lines. These validated KASP markers could facilitate faster breeding of new varieties with desired dormancy using marker-assisted early generation selection.
 
Publisher MDPI
 
Date 2022-02-06
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights cc_attribution
 
Identifier http://oar.icrisat.org/12598/1/Agronomy_12_1-18_2022.pdf
Bomireddy, D and Gangurde, S S and Variath, M T and Janila, P and Manohar, S S and Sharma, V and Parmar, S and Deshmukh, D and Reddisekhar, M and Reddy, D M and Sudhakar, P and Reddy, B V B and Varshney, R K and Guo, B and Pandey, M K (2022) Discovery of Major Quantitative Trait Loci and Candidate Genes for Fresh Seed Dormancy in Groundnut. Agronomy (TSI), 12 (2). pp. 1-18. ISSN 2073-4395