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Role of cannabinoid CB1 receptors in the proconvulsant effect of Apelin-13 on penicillin-induced epileptiform activity

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Title Role of cannabinoid CB1 receptors in the proconvulsant effect of Apelin-13 on penicillin-induced epileptiform activity
 
Creator Aycik, Fatma Banu
Ayyildiz, Mustafa
Agar, Erdal
 
Subject Brain electrocorticograhy
Cannabinoid CB1 receptor agonists
Epilepsy
Neuromodulators
 
Description 238-244
Epilepsy is a widespread neurological disorder. Many neurotransmitters, neuropeptides and neuromodulators have a
significant role in the epileptic activity. Apelin-13 and cannabinoid CB1 receptor agonist and antagonist have an effect in
the penicillin model of epilepsy. The relationship between apelin and epilepsy, and the apelin-cannabinoid relationship in
epilepsy is still not well understood. Thus, this study focuses on the relationship between apelin-13 and CB1 receptor in
experimental model of epilepsy. Penicillin injection was given intracortically (i.c.) for the development of epileptic seizures.
Ninety-one male Wistar rats were divided into 13 groups. CB1 receptor agonist ACEA (7.5 μg, intracerebroventricularly,
icv) and antagonist AM-251 (0.25 μg and 0.125 μg, icv) were administered to three different groups, two different doses of
apelin-13 (5 μg and 15 μg, icv) were applied and the interactions between these five groups of substances were evaluated.
Both apelin-13 (15 μg) and AM-251 (0.25 μg) raised the spike frequency of epileptiform activity separately. Application of
apelin-13 + AM-251 also increased the spike frequency of epileptiform activity beginning in the 30 min after apelin-13
application. When the non-effective dose of AM-251 and the effective dose of apelin-13 were administered together,
epileptic activity increased in the 20 min. ACEA reduced the epileptiform activity starting in the 50th min. apelin-13 and
ACEA administration in effective doses decreased epileptiform activity. The non-effective doses of AM-251, apelin-13 and
effective dose of ACEA decreased the epileptiform activity in the 50 min. Application of non-effective doses of apelin and
AM-251 together does not induce any additional proconvulsant activity, and CB1 receptor agonist, ACEA reversed the
proconvulsant activity of apelin-13. These results suggest that they utilize different receptors to begin their own effects by
increasing intracellular Ca2+ in epilepsy. Considering that apelin-13 is an endogenous substance known for its
neuroprotective properties, the proconvulsant effect of apelin-13 in the presented study is remarkable.
 
Date 2024-03-27T05:57:13Z
2024-03-27T05:57:13Z
2024-04
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/63624
https://doi.org/10.56042/ijeb.v62i04.655
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.62(04) [April 2024]