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Modulations in antioxidant defence system and expression of genes involved in antioxidant and apoptosis pathway helps ward-off heat stress incows reared in hot-arid ambience of Thar desert

Indian Agricultural Research Journals

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Title Modulations in antioxidant defence system and expression of genes involved in antioxidant and apoptosis pathway helps ward-off heat stress incows reared in hot-arid ambience of Thar desert
 
Creator GUJAR, GAYATRI
KUMAR, VIJAY
SODHI, MONIKA
VIVEK, PRINCE
TIWARI, MANISH
MUKESH, MANISHI
 
Subject Antioxidants, Apoptosis, Bos indicus, Gene expression, Seasonal study, Temperature humidity index, Thermotolerance
 
Description The present study was designed to assess the seasonal perturbations in key antioxidant parameters: ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) scavenging activity, lipid per oxidation (LPO), uric acid and reduced glutathione (GSH) assay along with expression profile of genes that regulate the antioxidant pathway: manganese superoxide dismutase (SOD2), Glutathione Peroxidase 1 (GPX1) and apoptosis pathway: B-cell lymphoma 2 (BCL2) and Bcl-2-associated X protein (BAX) in the two crucial indicine cattle breeds- Sahiwal and Kankrej. The study was carried out in the hot-arid region of Rajasthan across four seasons, wherein, the meteorological parameters were recorded across seasons to calculate the prevailing temperature-humidity index (THI) to assess the extent of heat load on cows. The study revealed huge diurnal variations in the temperature across four seasons, while the THI during summer and hot-humid seasons was well in excess of 80, highlighting significant heat stress on cows. All the key antioxidant parameters, except GSH registered an increase with the incremental THI. The transcriptional profile of SOD2 and GPX1 mRNA expression revealed significantly higher expression during the higher THI months of both summer and hot-humid seasons. The expression pattern of BAX and BCL2 mRNA in PBMCs of Sahiwal cows revealed an initial induction during the month of summer season with significantly higher expression, followed by a decline by the hot-humid season. The findings highlight a crucial role played by antioxidant defence and genes regulating apoptosis pathway in thermotolerance of indicine cattle breeds.
 
Publisher Indian Council of Agricultural Research
 
Date 2024-04-04
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/132953
10.56093/ijans.v94i4.132953
 
Source The Indian Journal of Animal Sciences; Vol. 94 No. 4 (2024); 333–341
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/132953/54267
 
Rights Copyright (c) 2024 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0