Record Details

Impact of varying doses of Moringa leaf extract supplementation in the cryopreservation media on sperm quality, antioxidant capacity and antimicrobial activity of frozen-thawed buck spermatozoa

Indian Agricultural Research Journals

View Archive Info
 
 
Field Value
 
Title Impact of varying doses of Moringa leaf extract supplementation in the cryopreservation media on sperm quality, antioxidant capacity and antimicrobial activity of frozen-thawed buck spermatozoa
 
Creator GANGWAR, CHETNA
KUMAR, ASHOK
GURURAJ, K
MISHRA, A K
RANJAN, RAVI
KUMAR, MANISH
RAI, ALOK
MITTAL, NIKITA
 
Subject Antimicrobial potential, Antioxidant, Buck semen, Fertility, Moringa oleifera
 
Description The current investigation was planned to evaluate the effect of Moringa leaves also called Moringa oleifera (M. oleifera) aqueous extract on buck semen quality during cryopreservation, and its antimicrobial potential against the Escherichia coli (E. coli) isolated from the semen samples. Semen was collected from 8 Jakhrana bucks, and from each buck, 8 ejaculates were collected. Semen samples were subjected to bacteriological studies and pathogenic coli were isolated from semen samples. Then, various concentrations of M. oleifera extract were evaluated for its antimicrobial potential. Good quality semen samples were pooled during each collection. Pooled semen samples were then divided into 4 equal parts, and diluted in TRIS buffer containing different concentration of M. oleifera leaf aqueous extract (Different groups, i.e. T1-50 mg, T2-100 mg, T3-200 mg, C-0 mg of M. oleifera aqueous extract in 100 ml TRIS Buffer). Semen samples were evaluated for various sperm characteristics after cryopreservation. oleifera aqueous extract supplemented groups showed significant enhancement in sperm viability, sperm motility, acrosomal integrity and plasma membrane integrity. The treatment significantly reduced the lipid peroxidation in supplemented groups and M. oleifera extract shows the potent antimicrobial activity against the E. coli isolated from buck semen.
 
Publisher Indian Council of Agricultural Research
 
Date 2024-04-04
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/146821
10.56093/ijans.v94i4.146821
 
Source The Indian Journal of Animal Sciences; Vol. 94 No. 4 (2024); 362–368
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/146821/54271
https://epubs.icar.org.in/index.php/IJAnS/article/view/146821/54272
 
Rights Copyright (c) 2024 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0