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ANALYSIS OF CAROTENOIDS METABOLISMGENES LANDSCAPE IN POTATO AND CLONING OF ZEAXANTHIN EPOXIDASE GENE

Indian Agricultural Research Journals

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Title ANALYSIS OF CAROTENOIDS METABOLISMGENES LANDSCAPE IN POTATO AND CLONING OF ZEAXANTHIN EPOXIDASE GENE
 
Creator Dutt, Som
Devi, Kalpana
Chauhan, Mamta
Raigond, Pinky
Kumar, Dharmendra
Lal, Milan Kumar
Jaiswal, Arvind Kumar
Bandana
Kumar, Devendra
Luthra, Satish Kumar
Kumar, Dinesh
Singh, Brajesh
 
Subject Genome-wide
genome editing
introns
gene structure
genetic engineering
 
Description Genome-wide identification of all the genes involved in carotenoids biosynthesis were carried out in silicousing potato genome sequence. Of the 12 genes analysed [geranylgeranyl pyrophosphate synthase (GGPS), phytoene synthase(PSY), f-carotene desaturase (ZDS), carotenoid isomerise (CRTISO), lycopene ε-cyclase (LCY-ε), lycopene β-cyclase (LCY-β),β-carotene hydroxylase (CHY-β), violaxanthin de-epoxidase (VDE), zeaxanthin epoxidase (ZEP), neoxanthin synthase (NXS),carotenoid cleavage dioxygenase (CCD), 9-cisepoxycarotenoids dioxygenase (NCED)]; three genes namely ZDS, CRTISOand LCY-ε was found as single gene, on chromosome 1, 10 and 12, respectively. GGPS (chromosomes 4 & 11), LCY-β(chromosomes 4 & 10), CHY-β (chromosomes 3& 6) had two genes each. VDE (chromosome 4), NXS (chromosomes 2, 3 &6) and NCED (chromosome 5, 7 & 8) showed presence of 3, 4 and 5 genes, respectively. CCD (chromosome 1 7 & 8) andPSY (chromosomes 2, 3, 4, 8 & 12) had six genes each in potato genome. These carotenoid biosynthesis genes differed interms of number of introns, length of open reading frame, molecular weight and isoelectric points of deduced proteins,secondary structure composition etc. This information generated about genes and proteins of carotenoids biosynthesispathway in potato may be very crucial and of vital utility for genome editing mediated interventions for enhancing total/targeted carotenoids content in potato. Further, ZEP was cloned full length. The complete ORF of cloned ZEP was 2010nucleotides long and encoding a protein having 669 amino acids (NCBI acc no. MK852682). It exhibited variations at 9 aminoacid positions when compared with the one available in potato genome sequence database. These variations at 9 aminoacids might provide some uniqueness to the cloned gene which might be involved in regulation of carotenoid biosynthesispathway especially beta-carotene, violaxanthin, zeaxanthin carotenoids etc.
 
Publisher Indian Potato Association (IPA)
 
Date 2024-04-04
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/PotatoJ/article/view/138194
 
Source Potato Journal; Vol. 50 No. 2 (2023): Potato Journal
0973-5909
0970-8235
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/PotatoJ/article/view/138194/54281
 
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https://creativecommons.org/licenses/by-nc-sa/4.0