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Karyotype Differentiation in Cultivated Chickpea Revealed by Oligopainting Fluorescence in situ Hybridization

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Relation http://oar.icrisat.org/12614/
https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2021.791303/full
https://doi.org/10.3389/fpls.2021.791303
 
Title Karyotype Differentiation in Cultivated Chickpea Revealed by Oligopainting Fluorescence in situ Hybridization
 
Creator Doležalová, A
Sládeková, L
Šimoníková, D
Holušová, K
Karafiátová, M
Varshney, R K
Doležel, J
Hřibová, E
 
Subject Chickpea
 
Description Chickpea (Cicer arietinum L.) is one of the main sources of plant proteins in the Indian subcontinent and West Asia, where two different morphotypes, desi and kabuli, are grown. Despite the progress in genome mapping and sequencing, the knowledge of the chickpea genome at the chromosomal level, including the long-range molecular chromosome organization, is limited. Earlier cytogenetic studies in chickpea suffered from a limited number of cytogenetic landmarks and did not permit to identify individual chromosomes in the metaphase spreads or to anchor pseudomolecules to chromosomes in situ. In this study, we developed a system for fast molecular karyotyping for both morphotypes of cultivated chickpea. We demonstrate that even draft genome sequences are adequate to develop oligo-fluorescence in situ hybridization (FISH) barcodes for the identification of chromosomes and comparative analysis among closely related chickpea genotypes. Our results show the potential of oligo-FISH barcoding for the identification of structural changes in chromosomes, which accompanied genome diversification among chickpea cultivars. Moreover, oligo-FISH barcoding in chickpea pointed out some problematic, most probably wrongly assembled regions of the pseudomolecules of both kabuli and desi reference genomes. Thus, oligo-FISH appears as a powerful tool not only for comparative karyotyping but also for the validation of genome assemblies.
 
Publisher Frontiers Media
 
Date 2022-01-25
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights cc_attribution
 
Identifier http://oar.icrisat.org/12614/1/Frontiers%20in%20Plant%20Science_12_1-11_2021.pdf
Doležalová, A and Sládeková, L and Šimoníková, D and Holušová, K and Karafiátová, M and Varshney, R K and Doležel, J and Hřibová, E (2022) Karyotype Differentiation in Cultivated Chickpea Revealed by Oligopainting Fluorescence in situ Hybridization. Frontiers in Plant Science (TSI), 12. pp. 1-11. ISSN 1664-462X