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Isolation and PCR amplification of genomic DNA from dried capsules of cardamon ( Elettaria cardamomum M.)

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Title Isolation and PCR amplification of genomic DNA from dried capsules of cardamon ( Elettaria cardamomum M.)
Not Available
 
Creator S Syamkumar
J Mrudula
B Sasikumar
 
Subject Elettaria cardamomum
cardamom
dna isolation
RAPD PCR
 
Description Not Available
Cardamom is an important spice, condiment and medicine, and international commodity. DNA-based molecular profiling will be aid in protecting the intellectual property rights of those who trade cardamom on the world market. Commercial cardamom has so far proven recalcitrant to traditional DNA extraction methods. In this paper we report a protocol for the isolation of amplifiable genomic DNA from traded cardamom. The method involves a modified CTAB (hexadecyltrimethylammonium bromide) extraction step, followed by a purification step to remove polysaccharides, proteins, and polyphenols, which are abundant in storage tissue such as cardamom capsules. The yield of DNA was 6–7 μg g−1 tissue. Spectrophotometric and electrophoretic analysis indicated that the isolated DNA was highly pure and of high molecular weight. The isolated DNA could be amplified using different random decamer primers. The protocol has trade implications as it will help in the PCR-based characterisation of traded cardamom. This protocol can be further extended to develop Sequence Characterised Amplified Regions (SCAR) markers for profiling cardamoms.
Not Available
 
Date 2024-04-12T14:51:05Z
2024-04-12T14:51:05Z
2005-12
 
Type Research Paper
 
Identifier Syamkumar, S., Mrudula, J. and B. Sasikumar (2005). Isolation and amplification of genomic DNA from traded cardamom. Plant Molecular biology Reporter 23: l a- j e.
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/82013
 
Language English
 
Relation Not Available;
 
Publisher ICAR- Indian institute of spices research,Kozhikode, Kerala India