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Method for isolation of soil DNA and PCR based detection of ginger wilt pathogen, Ralstonia solanacearum

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Title Method for isolation of soil DNA and PCR based detection of ginger wilt pathogen, Ralstonia solanacearum
Not Available
 
Creator A. KUMAR
M. ANANDARAJ
 
Subject PCR
specific primers
Ralstonia solanacearum
ginger
 
Description Not Available
An efficient DNA isolation protocol and PCR based detection of bacterial pathogen in soil are
described here. The use of this DNA isolation protocol and PCR based method using universal Ralstonia solanacearum specific primer offer a rapid method for unambiguous detection of this pathogen in soil which can be employed for monitoring soil. The PCR based assay could detect the pathogen at a concentration of 103-104cells per gram of soil.
Not Available
 
Date 2024-04-17T15:06:00Z
2024-04-17T15:06:00Z
2006
 
Type Research Paper
 
Identifier Kumar, A and M. Anandaraj (2006) Method for isolation of soil DNA and PCR based detection of ginger wilt pathogen, Ralstonia solanacearum. Indian Phytopathology 59: 154-160
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/82122
 
Language English
 
Relation Not Available;
 
Publisher ICAR- Indian institute of spices research,Kozhikode, Kerala India