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Development of an intelligent laser biospeckle system for early detection and classification of soybean seeds infected with seed-borne fungal pathogen (Colletotrichum truncatum)

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Title Development of an intelligent laser biospeckle system for early detection and classification of soybean seeds infected with seed-borne fungal pathogen (Colletotrichum truncatum)
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Creator Puneet Singh, Amit Chatterjee, Laxman S Rajput, Santosh Rana, Sanjeev Kumar, Vennampally Nataraj, Vimal Bhatia, Shashi Prakash
 
Subject Agriculture, Biospeckle analysis, early detection, laser, machine learning
 
Description Project code: CRG/2018/002697 of DST Project name:Development of Laser Biospeckle Technique for Applications in Agriculture
There is a need for developing rapid and non-destructive techniques for the early detection of seed-borne fungal pathogen because they can be an essential step towards adopting effective disease control measures. Existing techniques for detecting seed-borne diseases have poor sensitivity towards early stages of pathogen development (i.e., when seeds are asymptomatic) and they are also expensive, time-consuming, complex, require mycological skills and destructive testing operations. Aiming at overcoming the above limitations of the existing techniques, a novel laser biospeckle based method is proposed for early detection of seed-borne fungal infection in conjunction with machine learning. Soybean seeds infected by low concentrations (102-106 spores ml−1) of Colletotrichum truncatum were analysed by using full field biospeckle analysis to establish the possible relationship between biological activity in early stages of pathogen infection, with and without the use of frequency filtering. The results demonstrate that the biospeckle activity (BA), for both, raw and frequency filtered data was significantly high (p < 0.05) for the diseased seeds even for low inoculum concentrations. Moreover, the amplitude values of mid frequency spectral components for diseased seeds were higher than those of lower and higher spectral components which correspond to the BA of fungal infected seeds. Several classical machine learning algorithms were trained to model the response of healthy and diseased samples after parameter optimisation. Obtained results showed that k-nearest neighbour (k-NN), decision tree (DT), and artificial neural network (ANN) based predictive models presented strong robustness and high performance with overall accuracy reaching up to 96.94% for classifying diseased seeds.
DST
 
Date 2024-04-18T11:28:49Z
2024-04-18T11:28:49Z
2021-11-01
 
Type Article
 
Identifier Puneet Singh, Amit Chatterjee, Laxman S Rajput, Santosh Rana, Sanjeev Kumar, Vennampally Nataraj, Vimal Bhatia, Shashi Prakash 2021 Development of an intelligent laser biospeckle system for early detection and classification of soybean seeds infected with a seedborne fungal pathogen (Colletotrichumtruncatum), Biosystems Engineering, Volume 212, Pages 442-457.
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http://krishi.icar.gov.in/jspui/handle/123456789/82191
 
Language English
 
Relation Not Available;
 
Publisher Academic Press